测序及更多信息： https://pan.baidu.com/s/1FM6iku2JAhKW3QAmE9Hzmw?pwd=hedg

采购地址：淘宝店铺地址

• PURPOSE

  Expresses high specificity SpCas9. Px330-like plasmid.

• Bacterial Resistance(s)
  Ampicillin, 100 μg/mL
• Growth Temperature
  37°C
• Growth Strain(s)
  Stbl3
• Copy number
  High Copy

• enhanced specificity Cas9 (1.1)
• Alt name
  eSpCas9(1.1)
• Species
  S. pyogenes
• Mutation
  K848A, K1003A, & R1060A
• PromoterCBh
• Tag / Fusion Protein
  • 3xFLAG (N terminal on insert)

参考文献

Rationally engineered Cas9 nucleases with improved specificity. Slaymaker IM, Gao L, Zetsche B, Scott DA, Yan WX, Zhang F. Science. 2015 Dec 1. pii: aad5227. 10.1126/science.aad5227 PubMed 26628643