原核编辑质粒

pC013-Twinstrep-SUMO-huLwCas13a (Plasmid #90097)原核表达CAS13a 蛋白strep标签C2C2

发布时间:2019-12-10 11:13:14 | 浏览次数：

测序及更多信息：

采购地址：淘宝店铺地址

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切割RNA的Cas13a酶(之前称作C2c2)能够特异性地降低哺乳动物细胞中的内源性RNA和报告RNA水平

pC013-Twinstrep-SUMO-huLwCas13a

载体名称:	pC013-Twinstrep-SUMO-huLwCas13a
质粒类型:	大肠杆菌基因表达
高拷贝/低拷贝:	高拷贝
启动子:	T7
克隆方法:	多克隆位点，限制性内切酶
载体大小:	9542 bp
5' 测序引物及序列:	T7: 5'-TAATACGACTCACTATAGGG-3'
	T7t: 5'-GCTAGTTATTGCTCAGCGG-3'
载体标签:	N-HIS,N-SUMO,N-Twinstrep
载体抗性:	氨苄青霉素


组成型/诱导型:	诱导型
病毒/非病毒:	非病毒
备注:

pC013-Twinstrep-SUMO-huLwCas13a

(Plasmid #90097)

Twinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.

Gene/Insert name
LwCas13a
Alt name
LwC2c2
Alt name
Cas13a
Alt name
C2c2
Species
Synthetic; Leptotrichia wadeii
Insert Size (bp)
3280
Tag / Fusion Protein
- 6x His Tag/Twin Strep/SUMO (N terminal on insert)

参考文献

Nucleic acid detection with CRISPR-Cas13a/C2c2. Gootenberg JS, Abudayyeh OO, Lee JW, Essletzbichler P, Dy AJ, Joung J, Verdine V, Donghia N, Daringer NM, Freije CA, Myhrvold C, Bhattacharyya RP, Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, Collins JJ, Zhang F. Science. 2017 Apr 13. pii: eaam9321. doi: 10.1126/science.aam9321.10.1126/science.aam9321 PubMed 28408723