测序及更多信息： https://pan.baidu.com/s/1azl_djtu24oGI8PhbDIDXA?pwd=hedg

采购地址：淘宝店铺地址

• PURPOSE

  Mammalian expression vector for xCas9(3.7)-ABE
• DEPOSITING LAB

  David Liu

• Gene/Insert name
  xCas9(3.7)-ABE(7.10)
• Alt name
  TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nxCas9(3.7)-NLS
• Species
  Synthetic
• Insert Size (bp)
  5361
• Mutation
  A262T, R324L, S409I, E480K, E543D, M694I, E1219V
• Tag / Fusion Protein
  • NLS (N terminal on insert)

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Hu JH, Miller SM, Geurts MH, Tang W, Chen L, Sun N, Zeina CM, Gao X, Rees HA, Lin Z, Liu DR. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. 10.1038/nature26155 PubMed 29512652

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