| pGL4.18[luc2P/Neo] 信号通路报告载体 | ||||||||||||||||||||||||||||||
| 发布时间:2018-05-27 20:45:25 | 浏览次数： | ||||||||||||||||||||||||||||||
 |
||||||||||||||||||||||||||||||
测序及更多信息:https://pan.baidu.com/s/1_8Ughx08VsESQNYk7YgX-w 提取码：l1ey
采购地址：淘宝店铺地址
公司官网 http://hedgehogbio.com http://hedgehogbio.com
载体名称:
pGL4.18 、pGL4.18[luc2P/Neo]
质粒类型:
高拷贝/低拷贝:
高
启动子:
无启动子
克隆方法:
多克隆位点，限制性内切酶
载体大小:
5727bp
5' 测序引物及序列:
RVP3：CTAGCAAAATAGGCTGTCCC
3' 测序引物及序列:
--
载体标签:
--
载体抗性:
氨苄青霉素（Ampicillin）
筛选标记:
新霉素Neomycin
备注:
pGL4.18载体含Luciferase荧光素酶报告基因，无启动子，用于研究目的启动子的功能。
稳定性:
--
组成型:
组成型
病毒/非病毒:
非病毒
1 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAAGATC TGGCCTCGGC 61 GGCCAAGCTT GGCAATCCGG TACTGTTGGT AAAGCCACCA TGGAAGATGC CAAAAACATT 121 AAGAAGGGCC CAGCGCCATT CTACCCACTC GAAGACGGGA CCGCCGGCGA GCAGCTGCAC 181 AAAGCCATGA AGCGCTACGC CCTGGTGCCC GGCACCATCG CCTTTACCGA CGCACATATC 241 GAGGTGGACA TTACCTACGC CGAGTACTTC GAGATGAGCG TTCGGCTGGC AGAAGCTATG 301 AAGCGCTATG GGCTGAATAC AAACCATCGG ATCGTGGTGT GCAGCGAGAA TAGCTTGCAG 361 TTCTTCATGC CCGTGTTGGG TGCCCTGTTC ATCGGTGTGG CTGTGGCCCC AGCTAACGAC 421 ATCTACAACG AGCGCGAGCT GCTGAACAGC ATGGGCATCA GCCAGCCCAC CGTCGTATTC 481 GTGAGCAAGA AAGGGCTGCA AAAGATCCTC AACGTGCAAA AGAAGCTACC GATCATACAA 541 AAGATCATCA TCATGGATAG CAAGACCGAC TACCAGGGCT TCCAAAGCAT GTACACCTTC 601 GTGACTTCCC ATTTGCCACC CGGCTTCAAC GAGTACGACT TCGTGCCCGA GAGCTTCGAC 661 CGGGACAAAA CCATCGCCCT GATCATGAAC AGTAGTGGCA GTACCGGATT GCCCAAGGGC 721 GTAGCCCTAC CGCACCGCAC CGCTTGTGTC CGATTCAGTC ATGCCCGCGA CCCCATCTTC 781 GGCAACCAGA TCATCCCCGA CACCGCTATC CTCAGCGTGG TGCCATTTCA CCACGGCTTC 841 GGCATGTTCA CCACGCTGGG CTACTTGATC TGCGGCTTTC GGGTCGTGCT CATGTACCGC 901 TTCGAGGAGG AGCTATTCTT GCGCAGCTTG CAAGACTATA AGATTCAATC TGCCCTGCTG 961 GTGCCCACAC TATTTAGCTT CTTCGCTAAG AGCACTCTCA TCGACAAGTA CGACCTAAGC 1021 AACTTGCACG AGATCGCCAG CGGCGGGGCG CCGCTCAGCA AGGAGGTAGG TGAGGCCGTG 1081 GCCAAACGCT TCCACCTACC AGGCATCCGC CAGGGCTACG GCCTGACAGA AACAACCAGC 1141 GCCATTCTGA TCACCCCCGA AGGGGACGAC AAGCCTGGCG CAGTAGGCAA GGTGGTGCCC 1201 TTCTTCGAGG CTAAGGTGGT GGACTTGGAC ACCGGTAAGA CACTGGGTGT GAACCAGCGC 1261 GGCGAGCTGT GCGTCCGTGG CCCCATGATC ATGAGCGGCT ACGTTAACAA CCCCGAGGCT 1321 ACAAACGCTC TCATCGACAA GGACGGCTGG CTGCACAGCG GCGACATCGC CTACTGGGAC 1381 GAGGACGAGC ACTTCTTCAT CGTGGACCGG CTGAAGAGCC TGATCAAATA CAAGGGCTAC 1441 CAGGTAGCCC CAGCCGAACT GGAGAGCATC CTGCTGCAAC ACCCCAACAT CTTCGACGCC 1501 GGGGTCGCCG GCCTGCCCGA CGACGATGCC GGCGAGCTGC CCGCCGCAGT CGTCGTGCTG 1561 GAACACGGTA AAACCATGAC CGAGAAGGAG ATCGTGGACT ATGTGGCCAG CCAGGTTACA 1621 ACCGCCAAGA AGCTGCGCGG TGGTGTTGTG TTCGTGGACG AGGTGCCTAA AGGACTGACC 1681 GGCAAGTTGG ACGCCCGCAA GATCCGCGAG ATTCTCATTA AGGCCAAGAA GGGCGGCAAG 1741 ATCGCCGTGA ATTCTCACGG CTTCCCTCCC GAGGTGGAGG AGCAGGCCGC CGGCACCCTG 1801 CCCATGAGCT GCGCCCAGGA GAGCGGCATG GATAGACACC CTGCTGCTTG CGCCAGCGCC 1861 AGGATCAACG TCTAAGGCCG CGACTCTAGA GTCGGGGCGG CCGGCCGCTT CGAGCAGACA 1921 TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT 1981 TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC 2041 AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG 2101 TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTAAAAT CGATAAGGAT CCGTTTGCGT 2161 ATTGGGCGCT CTTCCGCTGA TCTGCGCAGC ACCATGGCCT GAAATAACCT CTGAAAGAGG 2221 AACTTGGTTA GCTACCTTCT GAGGCGGAAA GAACCAGCTG TGGAATGTGT GTCAGTTAGG 2281 GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA 2341 GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT 2401 GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC 2461 TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA 2521 GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG 2581 CCTAGGCTTT TGCAAAAAGC TCGATTCTTC TGACACTAGC GCCACCATGA TCGAACAAGA 2641 CGGCCTCCAT GCTGGCAGTC CCGCAGCTTG GGTCGAACGC TTGTTCGGGT ACGACTGGGC 2701 CCAGCAGACC ATCGGATGTA GCGATGCGGC CGTGTTCCGT CTAAGCGCTC AAGGCCGGCC 2761 CGTGCTGTTC GTGAAGACCG ACCTGAGCGG CGCCCTGAAC GAGCTTCAAG ACGAGGCTGC 2821 CCGCCTGAGC TGGCTGGCCA CCACCGGCGT ACCCTGCGCC GCTGTGTTGG ATGTTGTGAC 2881 CGAAGCCGGC CGGGACTGGC TGCTGCTGGG CGAGGTCCCT GGCCAGGATC TGCTGAGCAG 2941 CCACCTTGCC CCCGCTGAGA AGGTTTCTAT CATGGCCGAT GCAATGCGGC GCCTGCACAC 3001 CCTGGACCCC GCTACCTGCC CCTTCGACCA CCAGGCTAAG CATCGGATCG AGCGTGCTCG 3061 GACCCGCATG GAGGCCGGCC TGGTGGACCA GGACGACCTG GACGAGGAGC ATCAGGGCCT 3121 GGCCCCCGCT GAACTGTTCG CCCGACTGAA AGCCCGCATG CCGGACGGTG AGGACCTGGT 3181 TGTCACACAC GGAGATGCCT GCCTCCCTAA CATCATGGTC GAGAATGGCC GCTTCTCCGG 3241 CTTCATCGAC TGCGGTCGCC TAGGAGTTGC CGACCGCTAC CAGGACATCG CCCTGGCCAC 3301 CCGCGACATC GCTGAGGAGC TTGGCGGCGA GTGGGCCGAC CGCTTCTTAG TCTTGTACGG 3361 CATCGCAGCT CCCGACAGCC AGCGCATCGC CTTCTACCGC TTGCTCGACG AGTTCTTTTA 3421 ATGATCTAGA ACCGGTCATG GCCGCAATAA AATATCTTTA TTTTCATTAC ATCTGTGTGT 3481 TGGTTTTTTG TGTGTTCGAA CTAGATGCTG TCGACCGATG CCCTTGAGAG CCTTCAACCC 3541 AGTCAGCTCC TTCCGGTGGG CGCGGGGCAT GACTATCGTC GCCGCACTTA TGACTGTCTT 3601 CTTTATCATG CAACTCGTAG GACAGGTGCC GGCAGCGCTC TTCCGCTTCC TCGCTCACTG 3661 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA 3721 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC 3781 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 3841 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 3901 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 3961 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 4021 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 4081 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 4141 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 4201 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 4261 GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 4321 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC 4381 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 4441 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 4501 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 4561 AGTAAACTTG GTCTGACAGC GGCCGCAAAT GCTAAACCAC TGCAGTGGTT ACCAGTGCTT 4621 GATCAGTGAG GCACCGATCT CAGCGATCTG CCTATTTCGT TCGTCCATAG TGGCCTGACT 4681 CCCCGTCGTG TAGATCACTA CGATTCGTGA GGGCTTACCA TCAGGCCCCA GCGCAGCAAT 4741 GATGCCGCGA GAGCCGCGTT CACCGGCCCC CGATTTGTCA GCAATGAACC AGCCAGCAGG 4801 GAGGGCCGAG CGAAGAAGTG GTCCTGCTAC TTTGTCCGCC TCCATCCAGT CTATGAGCTG 4861 CTGTCGTGAT GCTAGAGTAA GAAGTTCGCC AGTGAGTAGT TTCCGAAGAG TTGTGGCCAT 4921 TGCTACTGGC ATCGTGGTAT CACGCTCGTC GTTCGGTATG GCTTCGTTCA ACTCTGGTTC 4981 CCAGCGGTCA AGCCGGGTCA CATGATCACC CATATTATGA AGAAATGCAG TCAGCTCCTT 5041 AGGGCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCGGTG TTGTCGCTCA TGGTAATGGC 5101 AGCACTACAC AATTCTCTTA CCGTCATGCC ATCCGTAAGA TGCTTTTCCG TGACCGGCGA 5161 GTACTCAACC AAGTCGTTTT GTGAGTAGTG TATACGGCGA CCAAGCTGCT CTTGCCCGGC 5221 GTCTATACGG GACAACACCG CGCCACATAG CAGTACTTTG AAAGTGCTCA TCATCGGGAA 5281 TCGTTCTTCG GGGCGGAAAG ACTCAAGGAT CTTGCCGCTA TTGAGATCCA GTTCGATATA 5341 GCCCACTCTT GCACCCAGTT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCGGGGTG 5401 TGCAAAAACA GGCAAGCAAA ATGCCGCAAA GAAGGGAATG AGTGCGACAC GAAAATGTTG 5461 GATGCTCATA CTCGTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ACTAGTACGT 5521 CTCTCAAGGA TAAGTAAGTA ATATTAAGGT ACGGGAGGTA TTGGACAGGC CGCAATAAAA 5581 TATCTTTATT TTCATTACAT CTGTGTGTTG GTTTTTTGTG TGAATCGATA GTACTAACAT 5641 ACGCTCTCCA TCAAAACAAA ACGAAACAAA ACAAACTAGC AAAATAGGCT GTCCCCAGTG 5701 CAAGTGCAGG TGCCAGAACA TTTCTCT |
||||||||||||||||||||||||||||||
| 上一篇:pGL4.10 报告基因表达载体；信号通路报告载体 pGL4.10[luc2] 启动子 下一篇:pGL4.20-68bp |
