测序及更多信息:https://pan.baidu.com/s/1_8Ughx08VsESQNYk7YgX-w 提取码：l1ey 

采购地址：淘宝店铺地址

公司官网 http://hedgehogbio.com   http://hedgehogbio.com  

The pGL4.23[luc2/minP] Vector(a–e)encodes the luciferase reporter gene luc2 (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. The pGL4.23[luc2/minP] Vector contains a multiple cloning region for insertion of a response element of interest upstream of a minimal promoter and a luc2 gene. luc2 is a synthetically-derived luciferase sequence with humanized codon optimization. The vector backbone contains an ampicillin resistance gene to allow for selection in E. coli. 

全序列

1        GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAAGATC TGGCCTCGGC
61       GGCCAAGCTT AGACACTAGA GGGTATATAA TGGAAGCTCG ACTTCCAGCT TGGCAATCCG
121      GTACTGTTGG TAAAGCCACC ATGGAAGATG CCAAAAACAT TAAGAAGGGC CCAGCGCCAT
181      TCTACCCACT CGAAGACGGG ACCGCCGGCG AGCAGCTGCA CAAAGCCATG AAGCGCTACG
241      CCCTGGTGCC CGGCACCATC GCCTTTACCG ACGCACATAT CGAGGTGGAC ATTACCTACG
301      CCGAGTACTT CGAGATGAGC GTTCGGCTGG CAGAAGCTAT GAAGCGCTAT GGGCTGAATA
361      CAAACCATCG GATCGTGGTG TGCAGCGAGA ATAGCTTGCA GTTCTTCATG CCCGTGTTGG
421      GTGCCCTGTT CATCGGTGTG GCTGTGGCCC CAGCTAACGA CATCTACAAC GAGCGCGAGC
481      TGCTGAACAG CATGGGCATC AGCCAGCCCA CCGTCGTATT CGTGAGCAAG AAAGGGCTGC
541      AAAAGATCCT CAACGTGCAA AAGAAGCTAC CGATCATACA AAAGATCATC ATCATGGATA
601      GCAAGACCGA CTACCAGGGC TTCCAAAGCA TGTACACCTT CGTGACTTCC CATTTGCCAC
661      CCGGCTTCAA CGAGTACGAC TTCGTGCCCG AGAGCTTCGA CCGGGACAAA ACCATCGCCC
721      TGATCATGAA CAGTAGTGGC AGTACCGGAT TGCCCAAGGG CGTAGCCCTA CCGCACCGCA
781      CCGCTTGTGT CCGATTCAGT CATGCCCGCG ACCCCATCTT CGGCAACCAG ATCATCCCCG
841      ACACCGCTAT CCTCAGCGTG GTGCCATTTC ACCACGGCTT CGGCATGTTC ACCACGCTGG
901      GCTACTTGAT CTGCGGCTTT CGGGTCGTGC TCATGTACCG CTTCGAGGAG GAGCTATTCT
961      TGCGCAGCTT GCAAGACTAT AAGATTCAAT CTGCCCTGCT GGTGCCCACA CTATTTAGCT
1021     TCTTCGCTAA GAGCACTCTC ATCGACAAGT ACGACCTAAG CAACTTGCAC GAGATCGCCA
1081     GCGGCGGGGC GCCGCTCAGC AAGGAGGTAG GTGAGGCCGT GGCCAAACGC TTCCACCTAC
1141     CAGGCATCCG CCAGGGCTAC GGCCTGACAG AAACAACCAG CGCCATTCTG ATCACCCCCG
1201     AAGGGGACGA CAAGCCTGGC GCAGTAGGCA AGGTGGTGCC CTTCTTCGAG GCTAAGGTGG
1261     TGGACTTGGA CACCGGTAAG ACACTGGGTG TGAACCAGCG CGGCGAGCTG TGCGTCCGTG
1321     GCCCCATGAT CATGAGCGGC TACGTTAACA ACCCCGAGGC TACAAACGCT CTCATCGACA
1381     AGGACGGCTG GCTGCACAGC GGCGACATCG CCTACTGGGA CGAGGACGAG CACTTCTTCA
1441     TCGTGGACCG GCTGAAGAGC CTGATCAAAT ACAAGGGCTA CCAGGTAGCC CCAGCCGAAC
1501     TGGAGAGCAT CCTGCTGCAA CACCCCAACA TCTTCGACGC CGGGGTCGCC GGCCTGCCCG
1561     ACGACGATGC CGGCGAGCTG CCCGCCGCAG TCGTCGTGCT GGAACACGGT AAAACCATGA
1621     CCGAGAAGGA GATCGTGGAC TATGTGGCCA GCCAGGTTAC AACCGCCAAG AAGCTGCGCG
1681     GTGGTGTTGT GTTCGTGGAC GAGGTGCCTA AAGGACTGAC CGGCAAGTTG GACGCCCGCA
1741     AGATCCGCGA GATTCTCATT AAGGCCAAGA AGGGCGGCAA GATCGCCGTG TAATAATTCT
1801     AGAGTCGGGG CGGCCGGCCG CTTCGAGCAG ACATGATAAG ATACATTGAT GAGTTTGGAC
1861     AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG
1921     CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT
1981     TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA
2041     AATGTGGTAA AATCGATAAG GATCCGTCGA CCGATGCCCT TGAGAGCCTT CAACCCAGTC
2101     AGCTCCTTCC GGTGGGCGCG GGGCATGACT ATCGTCGCCG CACTTATGAC TGTCTTCTTT
2161     ATCATGCAAC TCGTAGGACA GGTGCCGGCA GCGCTCTTCC GCTTCCTCGC TCACTGACTC
2221     GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG
2281     GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA
2341     GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA
2401     CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
2461     ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
2521     TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
2581     CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
2641     CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
2701     AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
2761     TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC
2821     AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
2881     TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
2941     TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC
3001     TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT
3061     CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA
3121     AACTTGGTCT GACAGCGGCC GCAAATGCTA AACCACTGCA GTGGTTACCA GTGCTTGATC
3181     AGTGAGGCAC CGATCTCAGC GATCTGCCTA TTTCGTTCGT CCATAGTGGC CTGACTCCCC
3241     GTCGTGTAGA TCACTACGAT TCGTGAGGGC TTACCATCAG GCCCCAGCGC AGCAATGATG
3301     CCGCGAGAGC CGCGTTCACC GGCCCCCGAT TTGTCAGCAA TGAACCAGCC AGCAGGGAGG
3361     GCCGAGCGAA GAAGTGGTCC TGCTACTTTG TCCGCCTCCA TCCAGTCTAT GAGCTGCTGT
3421     CGTGATGCTA GAGTAAGAAG TTCGCCAGTG AGTAGTTTCC GAAGAGTTGT GGCCATTGCT
3481     ACTGGCATCG TGGTATCACG CTCGTCGTTC GGTATGGCTT CGTTCAACTC TGGTTCCCAG
3541     CGGTCAAGCC GGGTCACATG ATCACCCATA TTATGAAGAA ATGCAGTCAG CTCCTTAGGG
3601     CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCGGTGTTGT CGCTCATGGT AATGGCAGCA
3661     CTACACAATT CTCTTACCGT CATGCCATCC GTAAGATGCT TTTCCGTGAC CGGCGAGTAC
3721     TCAACCAAGT CGTTTTGTGA GTAGTGTATA CGGCGACCAA GCTGCTCTTG CCCGGCGTCT
3781     ATACGGGACA ACACCGCGCC ACATAGCAGT ACTTTGAAAG TGCTCATCAT CGGGAATCGT
3841     TCTTCGGGGC GGAAAGACTC AAGGATCTTG CCGCTATTGA GATCCAGTTC GATATAGCCC
3901     ACTCTTGCAC CCAGTTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC GGGGTGTGCA
3961     AAAACAGGCA AGCAAAATGC CGCAAAGAAG GGAATGAGTG CGACACGAAA ATGTTGGATG
4021     CTCATACTCG TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTACTA GTACGTCTCT
4081     CAAGGATAAG TAAGTAATAT TAAGGTACGG GAGGTATTGG ACAGGCCGCA ATAAAATATC
4141     TTTATTTTCA TTACATCTGT GTGTTGGTTT TTTGTGTGAA TCGATAGTAC TAACATACGC
4201     TCTCCATCAA AACAAAACGA AACAAAACAA ACTAGCAAAA TAGGCTGTCC CCAGTGCAAG
4261     TGCAGGTGCC AGAACATTTC TCT