• Purpose

  In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

Backbone

• Vector backbone
  pmScarlet_C1
• Backbone manufacturer
  Gadella Lab
• Backbone size w/o insert (bp)4671
• Vector type
  Mammalian Expression
• Selectable markers
  Neomycin (select with G418)

ene/Insert

• Gene/Insert name
  Giantin
• Alt name
  Golgin B1
• Species
  H. sapiens (human)
• Insert Size (bp)
  390
• Mutation
  aa 3131-3259
• Entrez Gene
  GOLGB1 (a.k.a. GCP, GCP372, GOLIM1)
• PromoterCMV

参考文献

mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M, Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr. Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074. 10.1038/nmeth.4074 PubMed 27869816