测序及更多信息： https://pan.baidu.com/s/1azl_djtu24oGI8PhbDIDXA?pwd=hedg

采购地址：淘宝店铺地址

(Empty Backbone) Lentiviral vector for expression of high-specificity eSpCas9(1.1) and sgRNA scaffold

• ene/Insert name
  enhanced specificity Cas9 (1.1)
• Alt name
  eSpCas9(1.1)
• Species
  Synthetic
• Insert Size (bp)
  4945
• PromoterEF-1a core
• Tag / Fusion Protein
  • 3xFLAG (N terminal on insert)

• Bacterial Resistance(s)
  Ampicillin, 100 μg/mL
• Growth Temperature
  37°C
• Growth Strain(s)
  NEB Stable
• Copy number
  High Copy

参考文献

The E3 Ubiquitin Ligase Mind Bomb 1 Controls Adenovirus Genome Release at the Nuclear Pore Complex. Bauer M, Flatt JW, Seiler D, Cardel B, Emmenlauer M, Boucke K, Suomalainen M, Hemmi S, Greber UF. Cell Rep. 2019 Dec 17;29(12):3785-3795.e8. doi: 10.1016/j.celrep.2019.11.064. 10.1016/j.celrep.2019.11.064 PubMed 31851912