测序及更多信息：https://pan.baidu.com/s/1qaC-NrcQaKlkiw_D-s9i6Q
提取码：vg72
采购地址：淘宝店铺地址
我们的官网 http://hedgehogbio.comhttp://hedgehogbio.com/
载体基本信息
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载体名称:
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pHHLV-H1-copGFP-T2A-Puro
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质粒类型:
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慢病毒系统载体，shRNA，RNAi
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高拷贝/低拷贝:
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高
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启动子:
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H1
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克隆方法:
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多克隆位点，限制性内切酶
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载体大小:
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7860bp
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5' 测序引物及序列:
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H1: tcgctatgtgttctgggaaa
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3' 测序引物及序列:
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载体标签:
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--
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载体抗性:
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Ampicillian
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筛选标记:
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copGFP，puro
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备注:
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克隆宿主菌为Stbl3
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稳定性:
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稳表达
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组成型:
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组成型
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病毒/非病毒:
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慢病毒
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全序列
1 GCCAAGCTGA CGCGTGTAGT CTTATGCAAT ACTCTTGTAG TCTTGCAACA TGGTAACGAT
61 GAGTTAGCAA CATGCCTTAC AAGGAGAGAA AAAGCACCGT GCATGCCGAT TGGTGGAAGT
121 AAGGTGGTAC GATCGTGCCT TATTAGGAAG GCAACAGACG GGTCTGACAT GGATTGGACG
181 AACCACTGAA TTGCCGCATT GCAGAGATAT TGTATTTAAG TGCCTAGCTC GATACATAAA
241 CGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC
301 ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT
361 GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG
421 CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC CAGAGGAGCT CTCTCGACGC
481 AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG GGCGGCGACT GGTGAGTACG
541 CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG GGTGCGAGAG CGTCAGTATT
601 AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT TAAGGCCAGG GGGAAAGAAA
661 AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC TAGAACGATT CGCAGTTAAT
721 CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC TGGGACAGCT ACAACCATCC
781 CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA CAGTAGCAAC CCTCTATTGT
841 GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT TAGACAAGAT AGAGGAAGAG
901 CAAAACAAAA GTAAGACCAC CGCACAGCAA GCGGCCGCTG ATCTTCAGAC CTGGAGGAGG
961 AGATATGAGG GACAATTGGA GAAGTGAATT ATATAAATAT AAAGTAGTAA AAATTGAACC
1021 ATTAGGAGTA GCACCCACCA AGGCAAAGAG AAGAGTGGTG CAGAGAGAAA AAAGAGCAGT
1081 GGGAATAGGA GCTTTGTTCC TTGGGTTCTT GGGAGCAGCA GGAAGCACTA TGGGCGCAGC
1141 GTCAATGACG CTGACGGTAC AGGCCAGACA ATTATTGTCT GGTATAGTGC AGCAGCAGAA
1201 CAATTTGCTG AGGGCTATTG AGGCGCAACA GCATCTGTTG CAACTCACAG TCTGGGGCAT
1261 CAAGCAGCTC CAGGCAAGAA TCCTGGCTGT GGAAAGATAC CTAAAGGATC AACAGCTCCT
1321 GGGGATTTGG GGTTGCTCTG GAAAACTCAT TTGCACCACT GCTGTGCCTT GGAATGCTAG
1381 TTGGAGTAAT AAATCTCTGG AACAGATTGG AATCACACGA CCTGGATGGA GTGGGACAGA
1441 GAAATTAACA ATTACACAAG CTTAATACAC TCCTTAATTG AAGAATCGCA AAACCAGCAA
1501 GAAAAGAATG AACAAGAATT ATTGGAATTA GATAAATGGG CAAGTTTGTG GAATTGGTTT
1561 AACATAACAA ATTGGCTGTG GTATATAAAA TTATTCATAA TGATAGTAGG AGGCTTGGTA
1621 GGTTTAAGAA TAGTTTTTGC TGTACTTTCT ATAGTGAATA GAGTTAGGCA GGGATATTCA
1681 CCATTATCGT TTCAGACCCA CCTCCCAACC CCGAGGGGAC CCGACAGGCC CGAAGGAATA
1741 GAAGAAGAAG GTGGAGAGAG AGACAGAGAC AGATCCATTC GATTAGTGAA CGGATCTCGA
1801 CGGTTAACTT TTAAAAGAAA AGGGGGGATT GGGGGGTACA GTGCAGGGGA AAGAATAGTA
1861 GACATAATAG CAACAGACAT ACAAACTAAA GAATTACAAA AACAAATTAC AAAATTCAAA
1921 ATTTTATCGA TACTAGTATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA
1981 GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA
2041 TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA
2101 TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC
2161 CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCG
2221 TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC TCCATAGAAG
2281 ATTCTAGACG CCACCATGGA GAGCGACGAG AGCGGCCTGC CCGCCATGGA GATCGAGTGC
2341 CGCATCACCG GCACCCTGAA CGGCGTGGAG TTCGAGCTGG TGGGCGGCGG AGAGGGCACC
2401 CCCAAGCAGG GCCGCATGAC CAACAAGATG AAGAGCACCA AAGGCGCCCT GACCTTCAGC
2461 CCCTACCTGC TGAGCCACGT GATGGGCTAC GGCTTCTACC ACTTCGGCAC CTACCCCAGC
2521 GGCTACGAGA ACCCCTTCCT GCACGCCATC AACAACGGCG GCTACACCAA CACCCGCATC
2581 GAGAAGTACG AGGACGGCGG CGTGCTGCAC GTGAGCTTCA GCTACCGCTA CGAGGCCGGC
2641 CGCGTGATCG GCGACTTCAA GGTGGTGGGC ACCGGCTTCC CCGAGGACAG CGTGATCTTC
2701 ACCGACAAGA TCATCCGCAG CAACGCCACC GTGGAGCACC TGCACCCCAT GGGCGATAAC
2761 GTGCTGGTGG GCAGCTTCGC CCGCACCTTC AGCCTGCGCG ACGGCGGCTA CTACAGCTTC
2821 GTGGTGGACA GCCACATGCA CTTCAAGAGC GCCATCCACC CCAGCATCCT GCAGAACGGG
2881 GGCCCCATGT TCGCCTTCCG CCGCGTGGAG GAGCTGCACA GCAACACCGA GCTGGGCATC
2941 GTGGAGTACC AGCACGCCTT CAAGACCCCC ATCGCCTTCG CCAGATCCCG CGCTCAGTCG
3001 TCCAATTCTG CCGTGGACGG CACCGCCGGA CCCGGCTCCA CCGGATCTCG CGAGGGCAGA
3061 GGAAGTCTTC TAACATGCGG TGACGTGGAG GAGAATCCCG GCCCTATGAC CGAGTACAAG
3121 CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCAGGG CCGTACGCAC CCTCGCCGCC
3181 GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGATC CGGACCGCCA CATCGAGCGG
3241 GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG
3301 GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG
3361 GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG
3421 CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG
3481 GCCACCGTCG GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC
3541 CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC
3601 CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC
3661 GAAGGACCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG CCTGA AATCAACCT
3721 CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG
3781 CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC
3841 ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT
3901 GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC
3961 ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG
4021 GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT
4081 GACAATTCCG TGGTGTTGTC GGGGAAATCA TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT
4141 GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG
4201 GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT CCGCCTTCGC
4261 CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC CTGGTACCTT TAAGACCAAT
4321 GACTTACAAG GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGGGGG GACTGGAAGG
4381 GCTAATTCAC TCCCAACGAT GTCAAGAATT GGAACGCTGA CGTCATCAAC CCGCTCCAAG
4441 GAATCGCGGG CCCAGTGTCA CTAGGCGGGA ACACCCAGCG CGCGTGCGCC CTGGCAGGAA
4501 GATGGCTGTG AGGGACAGGG GAGTGGCGCC CTGCAATATT TGCATGTCGC TATGTGTTCT
4561 GGGAAATCAC CATAAACGTG AAATGTCTTT GGATTTGGGA ATCTTATAAG TTCTGTATGA
4621 GACCACTTGG ATCCTCTGAA TTCTTCGATT CTGCTTTTTG CTTCTACTGG GTCTCTCTGG
4681 TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT
4741 CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT
4801 AACTAGAGAT CCCTCAGACC CTTTCAGTCA GTGTGGAAAA TCTCTAGCAG TAGTAGTTCA
4861 TGTCATCTTA TTATTCAGTA TTTATAACTT GCAAAGAAAT GAATATCAGA GAGTGAGAGG
4921 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA
4981 AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT
5041 TATCATGTCT GGCTCTAGCT ATCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC
5101 CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG
5161 AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG
5221 ACTTTTGCAG AGACCAAATT CGTAATCATG TCATAGCTGT TTCCTGTGTG AAATTGTTAT
5281 CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC
5341 TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA
5401 AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT
5461 ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG
5521 CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC
5581 GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG
5641 TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA
5701 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC
5761 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC
5821 CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG
5881 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC
5941 TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA
6001 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG
6061 AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG
6121 AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT
6181 GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA
6241 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
6301 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
6361 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC
6421 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA
6481 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA
6541 ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
6601 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
6661 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
6721 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT
6781 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC
6841 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
6901 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT
6961 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG
7021 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA
7081 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG
7141 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
7201 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
7261 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC
7321 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA
7381 TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT
7441 AAAAATAGGC GTATCACGAG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC
7501 CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC
7561 AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT
7621 GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA
7681 TGCGTAAGGA GAAAATACCG CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG
7741 GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT
7801 GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG
7861 GCCAGT
