• PURPOSE

  CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion

• Vector backbone
  pUC19
• Backbone size w/o insert (bp)2623
• Total vector size (bp)11804
• Vector type
  Yeast Expression, CRISPR, Synthetic Biology
• Selectable markers
  LEU2

GENE/INSERT 1

• Gene/Insert name
  Codon optimized Cas9 from S. pyogenes
• Species
  Synthetic
• Insert Size (bp)
  4140
• PromoterUAS1B8-TEF(136)

参考文献

Synthetic RNA polymerase III promoters facilitate high efficiency CRISPR-Cas9 mediated genome editing in Yarrowia lipolytica. Schwartz CM, Hussain MS, Blenner M, Wheeldon I. ACS Synth Biol. 2015 Dec 29. 10.1021/acssynbio.5b00162 PubMed 26714206