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pCold系列质粒

pCold TF pColdTF 原核 大肠杆菌蛋白表达载体
发布时间:2018-05-28 11:26:01 | 浏览次数:



测序及更多信息:https://pan.baidu.com/s/11A9pa_kKsMu_AcsxZRmZXw 
提取码:w3yc 

采购地址:淘宝店铺地址



pColdTF载体基本信息

出品公司:

Takara

载体名称:

pCold TF, pColdTF, pCold-TF

质粒类型:

大肠杆菌蛋白表达载体

表达水平:

启动子:

CSPA

克隆方法:

多克隆位点,限制性内切酶

载体大小:

5769 bp

5' 测序引物及序列:

pCold-TF-F1: 5'-CCACTTTCAACGAGCTGATG-3';
pCold-TF-F2: 5'-GCGAAAGTGACTGAAAAAG-3'

3' 测序引物及序列:

pCold-R: 5'-GGCAGGGATCTTAGATTCTG-3'

载体标签:

N-His, N-Trigger factor, N-HRV 3C, N-Thrombin, N-Factor Xa

载体抗性:

Ampicillin 氨苄

备注:

温度诱导,冷休克蛋白表达载体

稳定性:

瞬时表达 Transient

组成型:

诱导表达

病毒/非病毒:

非病毒


全序列

1        AAGGAATGGT GTGGCCGATT AATCATAAAT ATGAAAAATA ATTGTTGCAT CACCCGCCAA
61       TGCGTGGCTT AATGCACATC AAATTGTGAG CGGATAACAA TTTGATGTGC TAGCGCATAT
121      CCAGTGTAGT AAGGCAAGTC CCTTCAAGAG TTATCGTTGA TACCCCTCGT AGTGCACATT
181      CCTTTAACGC TTCAAAATCT GTAAAGCACG CCATATCGCC GAAAGGCACA CTTAATTATT
241      AAGAGGTAAT ACACCATGAA TCACAAAGTG CATCATCATC ATCATCACAT GCAAGTTTCA
301      GTTGAAACCA CTCAAGGCCT TGGCCGCCGT GTAACGATTA CTATCGCTGC TGACAGCATC
361      GAGACCGCTG TTAAAAGCGA GCTGGTCAAC GTTGCGAAAA AAGTACGTAT TGACGGCTTC
421      CGCAAGGGCA AAGTGCCAAT GAATATCGTT GCTCAGCGTT ATGGCGCGTC TGTACGCCAG
481      GACGTTCTGG GTGACCTGAT GAGCCGTAAC TTCATTGACG CCATCATTAA AGAAAAAATC
541      AATCCGGCTG GCGCACCGAC TTATGTTCCG GGCGAATACA AGCTGGGTGA AGACTTCACT
601      TACTCTGTAG AGTTTGAAGT TTATCCGGAA GTTGAACTGC AAGGTCTGGA AGCGATCGAA
661      GTTGAAAAAC CGATCGTTGA AGTGACCGAC GCTGACGTTG ACGGCATGCT GGATACTCTG
721      CGTAAACAGC AGGCGACCTG GAAAGAAAAA GACGGCGCTG TTGAAGCAGA AGACCGCGTG
781      ACCATCGACT TCACCGGTTC TGTAGACGGC GAAGAGTTCG AAGGCGGTAA AGCGTCTGAT
841      TTCGTACTGG CGATGGGCCA GGGTCGTATG ATCCCGGGCT TTGAAGACGG TATCAAAGGC
901      CACAAAGCTG GCGAAGAGTT CACCATCGAC GTGACCTTCC CGGAAGAATA CCACGCAGAA
961      AACCTGAAAG GTAAAGCAGC GAAATTCGCT ATCAACCTGA AGAAAGTTGA AGAGCGTGAA
1021     CTGCCGGAAC TGACCGCAGA GTTCATCAAA CGTTTCGGCG TTGAAGATGG TTCCGTAGAA
1081     GGTCTGCGCG CTGAAGTGCG TAAAAACATG GAGCGCGAGC TGAAGAGCGC CATCCGTAAC
1141     CGCGTTAAGT CTCAGGCGAT CGAAGGTCTG GTAAAAGCTA ACGACATCGA CGTACCGGCT
1201     GCGCTGATCG ACAGCGAAAT CGACGTTCTG CGTCGCCAGG CTGCACAGCG TTTCGGTGGC
1261     AACGAAAAAC AAGCTCTGGA ACTGCCGCGC GAACTGTTCG AAGAACAGGC TAAACGCCGC
1321     GTAGTTGTTG GCCTGCTGCT GGGCGAAGTT ATCCGCACCA ACGAGCTGAA AGCTGACGAA
1381     GAGCGCGTGA AAGGCCTGAT CGAAGAGATG GCTTCTGCGT ACGAAGATCC GAAAGAAGTT
1441     ATCGAGTTCT ACAGCAAAAA CAAAGAACTG ATGGACAACA TGCGCAATGT TGCTCTGGAA
1501     GAACAGGCTG TTGAAGCTGT ACTGGCGAAA GCGAAAGTGA CTGAAAAAGA AACCACTTTC
1561     AACGAGCTGA TGAACCAGCA GGCGTCCGCG GGTCTGGAAG TTCTGTTCCA GGGGCCCTCC
1621     GCGGGTCTGG TGCCACGCGG TAGTGGTGGT ATCGAAGGTA GGCATATGGA GCTCGGTACC
1681     CTCGAGGGAT CCGAATTCAA GCTTGTCGAC CTGCAGTCTA GATAGGTAAT CTCTGCTTAA
1741     AAGCACAGAA TCTAAGATCC CTGCCATTTG GCGGGGATTT TTTTATTTGT TTTCAGGAAA
1801     TAAATAATCG ATCGCGTAAT AAAATCTATT ATTATTTTTG TGAAGAATAA ATTTGGGTGC
1861     AATGAGAATG CGCAGGCCCT TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG
1921     ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA
1981     AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC
2041     ATCAGAGCAG ATTGTACTGA GAGTGCACCA TAAAATTGTA AACGTTAATA TTTTGTTAAA
2101     ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA
2161     AATCCCTTAT AAATCAAAAG AATAGCCCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA
2221     CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA
2281     GGGCGATGGC CCACTACGTG AACCATCACC CAAATCAAGT TTTTTGGGGT CGAGGTGCCG
2341     TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC
2401     GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC
2461     AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA
2521     GGGCGCGTAC TATGGTTGCT TTGACGTATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG
2581     AGAAAATACC GCATCAGGCG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA
2641     TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT
2701     AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC
2761     TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA
2821     AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA
2881     ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT
2941     TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG
3001     GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC
3061     ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA
3121     ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT
3181     TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG
3241     CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA
3301     AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG
3361     AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG
3421     CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG
3481     ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG
3541     AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG
3601     ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA
3661     TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT
3721     TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC
3781     TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC
3841     CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC
3901     CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC
3961     CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT
4021     CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT
4081     GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT
4141     ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT
4201     ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG
4261     CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT
4321     GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT
4381     TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC
4441     TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGAGATCC CGGTGCCTAA TGAGTGAGCT
4501     AACTTACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC
4561     AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCCAGG
4621     GTGGTTTTTC TTTTCACCAG TGAGACGGGC AACAGCTGAT TGCCCTTCAC CGCCTGGCCC
4681     TGAGAGAGTT GCAGCAAGCG GTCCACGCTG GTTTGCCCCA GCAGGCGAAA ATCCTGTTTG
4741     ATGGTGGTTA ACGGCGGGAT ATAACATGAG CTGTCTTCGG TATCGTCGTA TCCCACTACC
4801     GAGATATCCG CACCAACGCG CAGCCCGGAC TCGGTAATGG CGCGCATTGC GCCCAGCGCC
4861     ATCTGATCGT TGGCAACCAG CATCGCAGTG GGAACGATGC CCTCATTCAG CATTTGCATG
4921     GTTTGTTGAA AACCGGACAT GGCACTCCAG TCGCCTTCCC GTTCCGCTAT CGGCTGAATT
4981     TGATTGCGAG TGAGATATTT ATGCCAGCCA GCCAGACGCA GACGCGCCGA GACAGAACTT
5041     AATGGGCCCG CTAACAGCGC GATTTGCTGG TGACCCAATG CGACCAGATG CTCCACGCCC
5101     AGTCGCGTAC CGTCTTCATG GGAGAAAATA ATACTGTTGA TGGGTGTCTG GTCAGAGACA
5161     TCAAGAAATA ACGCCGGAAC ATTAGTGCAG GCAGCTTCCA CAGCAATGGC ATCCTGGTCA
5221     TCCAGCGGAT AGTTAATGAT CAGCCCACTG ACGCGTTGCG CGAGAAGATT GTGCACCGCC
5281     GCTTTACAGG CTTCGACGCC GCTTCGTTCT ACCATCGACA CCACCACGCT GGCACCCAGT
5341     TGATCGGCGC GAGATTTAAT CGCCGCGACA ATTTGCGACG GCGCGTGCAG GGCCAGACTG
5401     GAGGTGGCAA CGCCAATCAG CAACGACTGT TTGCCCGCCA GTTGTTGTGC CACGCGGTTG
5461     GGAATGTAAT TCAGCTCCGC CATCGCCGCT TCCACTTTTT CCCGCGTTTT CGCAGAAACG
5521     TGGCTGGCCT GGTTCACCAC GCGGGAAACG GTCTGATAAG AGACACCGGC ATACTCTGCG
5581     ACATCGTATA ACGTTACTGG TTTCACATTC ACCACCCTGA ATTGACTCTC TTCCGGGCGC
5641     TATCATGCCA TACCGCGAAA GGTTTTGCGC CATTCGATGG TGTCCGGGAT CTCGACGCTC
5701     TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC
5761     CGCCGCCGC




 
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 下一篇:pCold SUMO 大肠杆菌表达载体 温度诱导 质粒 蛋白表达载体

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