BACKBONE

• Vector backbone
  pCT
• Backbone size w/o insert (bp)6142
• Total vector size (bp)6456
• Vector type
  Yeast Expression
• Selectable markers
  TRP1

GENE/INSERT

• Gene/Insert name
  CD20
• Insert Size (bp)
  183
• Tags / Fusion Proteins
  • HA (N terminal on backbone)
  • c-myc (C terminal on backbone)

参考文献

Isolating and engineering human antibodies using yeast surface display. Chao G, Lau WL, Hackel BJ, Sazinsky SL, Lippow SM, Wittrup KD. Nat Protoc. 2006;1(2):755-68. 10.1038/nprot.2006.94 PubMed 17406305