测序及更多信息：https://pan.baidu.com/s/1qUMGmzHs3sum-9TQ-22pqg 密码：1ebu
采购地址：淘宝店铺地址
pPICZα A载体基本信息
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出品公司:
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Invitrogen
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载体名称:
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pPICZalpha
A, pPICZα A, pPICZalphaA, pPICZαA, pPICZaA, pPICZa-A
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质粒类型:
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毕赤酵母蛋白表达载体
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表达水平:
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高拷贝
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启动子:
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AOX1
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克隆方法:
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多克隆位点，限制性内切酶
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载体大小:
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3593
bp
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5' 测序引物及序列:
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5´
AOX1:5´-GACTGGTTCCAATTGACAAGC-3´
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3' 测序引物及序列:
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3´
AOX1:5´-GCAAATGGCATTCTGACATCC-3´
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载体标签:
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C-Myc,
C-His
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载体抗性:
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Zeocin 博来霉素
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筛选标记:
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HIS4
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备注:
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该载体本身含有signal alpha 分泌信号肽，该信号肽本身含有ATG启动子，所以插入基因可以不包含起始密码子ATG。
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稳定性:
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稳定 Stable
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组成型:
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组成型 Constitutive
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病毒/非病毒:
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非病毒
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全序列
1 AGATCTAACA TCCAAAGACG AAAGGTTGAA TGAAACCTTT TTGCCATCCG ACATCCACAG
61 GTCCATTCTC ACACATAAGT GCCAAACGCA ACAGGAGGGG ATACACTAGC AGCAGACCGT
121 TGCAAACGCA GGACCTCCAC TCCTCTTCTC CTCAACACCC ACTTTTGCCA TCGAAAAACC
181 AGCCCAGTTA TTGGGCTTGA TTGGAGCTCG CTCATTCCAA TTCCTTCTAT TAGGCTACTA
241 ACACCATGAC TTTATTAGCC TGTCTATCCT GGCCCCCCTG GCGAGGTTCA TGTTTGTTTA
301 TTTCCGAATG CAACAAGCTC CGCATTACAC CCGAACATCA CTCCAGATGA GGGCTTTCTG
361 AGTGTGGGGT CAAATAGTTT CATGTTCCCC AAATGGCCCA AAACTGACAG TTTAAACGCT
421 GTCTTGGAAC CTAATATGAC AAAAGCGTGA TCTCATCCAA GATGAACTAA GTTTGGTTCG
481 TTGAAATGCT AACGGCCAGT TGGTCAAAAA GAAACTTCCA AAAGTCGGCA TACCGTTTGT
541 CTTGTTTGGT ATTGATTGAC GAATGCTCAA AAATAATCTC ATTAATGCTT AGCGCAGTCT
601 CTCTATCGCT TCTGAACCCC GGTGCACCTG TGCCGAAACG CAAATGGGGA AACACCCGCT
661 TTTTGGATGA TTATGCATTG TCTCCACATT GTATGCTTCC AAGATTCTGG TGGGAATACT
721 GCTGATAGCC TAACGTTCAT GATCAAAATT TAACTGTTCT AACCCCTACT TGACAGCAAT
781 ATATAAACAG AAGGAAGCTG CCCTGTCTTA AACCTTTTTT TTTATCATCA TTATTAGCTT
841 ACTTTCATAA TTGCGACTGG TTCCAATTGA CAAGCTTTTG ATTTTAACGA CTTTTAACGA
901 CAACTTGAGA AGATCAAAAA ACAACTAATT ATTCGAAACG ATGAGATTTC CTTCAATTTT
961 TACTGCTGTT TTATTCGCAG CATCCTCCGC ATTAGCTGCT CCAGTCAACA CTACAACAGA
1021 AGATGAAACG GCACAAATTC CGGCTGAAGC TGTCATCGGT TACTCAGATT TAGAAGGGGA
1081 TTTCGATGTT GCTGTTTTGC CATTTTCCAA CAGCACAAAT AACGGGTTAT TGTTTATAAA
1141 TACTACTATT GCCAGCATTG CTGCTAAAGA AGAAGGGGTA TCTCTCGAGA AAAGAGAGGC
1201 TGAAGCTGAA TTCACGTGGC CCAGCCGGCC GTCTCGGATC GGTACCTCGA GCCGCGGCGG
1261 CCGCCAGCTT TCTAGAACAA AAACTCATCT CAGAAGAGGA TCTGAATAGC GCCGTCGACC
1321 ATCATCATCA TCATCATTGA GTTTGTAGCC TTAGACATGA CTGTTCCTCA GTTCAAGTTG
1381 GGCACTTACG AGAAGACCGG TCTTGCTAGA TTCTAATCAA GAGGATGTCA GAATGCCATT
1441 TGCCTGAGAG ATGCAGGCTT CATTTTTGAT ACTTTTTTAT TTGTAACCTA TATAGTATAG
1501 GATTTTTTTT GTCATTTTGT TTCTTCTCGT ACGAGCTTGC TCCTGATCAG CCTATCTCGC
1561 AGCTGATGAA TATCTTGTGG TAGGGGTTTG GGAAAATCAT TCGAGTTTGA TGTTTTTCTT
1621 GGTATTTCCC ACTCCTCTTC AGAGTACAGA AGATTAAGTG AGACCTTCGT TTGTGCGGAT
1681 CCCCCACACA CCATAGCTTC AAAATGTTTC TACTCCTTTT TTACTCTTCC AGATTTTCTC
1741 GGACTCCGCG CATCGCCGTA CCACTTCAAA ACACCCAAGC ACAGCATACT AAATTTTCCC
1801 TCTTTCTTCC TCTAGGGTGT CGTTAATTAC CCGTACTAAA GGTTTGGAAA AGAAAAAAGA
1861 GACCGCCTCG TTTCTTTTTC TTCGTCGAAA AAGGCAATAA AAATTTTTAT CACGTTTCTT
1921 TTTCTTGAAA TTTTTTTTTT TAGTTTTTTT CTCTTTCAGT GACCTCCATT GATATTTAAG
1981 TTAATAAACG GTCTTCAATT TCTCAAGTTT CAGTTTCATT TTTCTTGTTC TATTACAACT
2041 TTTTTTACTT CTTGTTCATT AGAAAGAAAG CATAGCAATC TAATCTAAGG GGCGGTGTTG
2101 ACAATTAATC ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA
2161 CCATGGCCAA GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG
2221 TCGAGTTCTG GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG
2281 GTGTGGTCCG GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG
2341 ACAACACCCT GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG
2401 AGGTCGTGTC CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC
2461 AGCCGTGGGG GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG
2521 CCGAGGAGCA GGACTGACAC GTCCGACGGC GGCCCACGGG TCCCAGGCCT CGGAGATCCG
2581 TCCCCCTTTT CCTTTGTCGA TATCATGTAA TTAGTTATGT CACGCTTACA TTCACGCCCT
2641 CCCCCCACAT CCGCTCTAAC CGAAAAGGAA GGAGTTAGAC AACCTGAAGT CTAGGTCCCT
2701 ATTTATTTTT TTATAGTTAT GTTAGTATTA AGAACGTTAT TTATATTTCA AATTTTTCTT
2761 TTTTTTCTGT ACAGACGCGT GTACGCATGT AACATTATAC TGAAAACCTT GCTTGAGAAG
2821 GTTTTGGGAC GCTCGAAGGC TTTAATTTGC AAGCTGGAGA CCAACATGTG AGCAAAAGGC
2881 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC
2941 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA
3001 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC
3061 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA
3121 TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG
3181 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC
3241 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA
3301 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT
3361 AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT
3421 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG
3481 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG
3541 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATC
