测序及更多信息： 链接：https://pan.baidu.com/s/1p4wJsVUreguOup76zRGBZg
提取码：gvvw
采购地址：淘宝店铺地址
pYES2/CT/α-Factor 载体
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质粒类型:
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酿酒酵母表达载体
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诱导方法:
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半乳糖
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启动子:
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GAL1
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5' 测序引物及序列:
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GAL1-F:
AATATACCTCTATACTTTAACGTC
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3' 测序引物及序列:
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CYC1-R: GCGTGAATGTAAGCGTGAC
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载体标签:
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C-His, C-V5
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载体抗性:
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Ampicillin (氨苄青霉素)
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筛选标记:
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URA3
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备注:
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利用半乳糖诱导蛋白在酿酒酵母中表达。
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一种酿酒酵母附加型分泌表达载体pYES2/CT/α-Factor的构建及鉴定
全序列
1 ACGGATTAGA AGCCGCCGAG CGGGTGACAG CCCTCCGAAG GAAGACTCTC CTCCGTGCGT
61 CCTCGTCTTC ACCGGTCGCG TTCCTGAAAC GCAGATGTGC CTCGCGCCGC ACTGCTCCGA
121 ACAATAAAGA TTCTACAATA CTAGCTTTTA TGGTTATGAA GAGGAAAAAT TGGCAGTAAC
181 CTGGCCCCAC AAACCTTCAA ATGAACGAAT CAAATTAACA ACCATAGGAT GATAATGCGA
241 TTAGTTTTTT AGCCTTATTT CTGGGGTAAT TAATCAGCGA AGCGATGATT TTTGATCTAT
301 TAACAGATAT ATAAATGCAA AAACTGCATA ACCACTTTAA CTAATACTTT CAACATTTTC
361 GGTTTGTATT ACTTCTTATT CAAATGTAAT AAAAGTATCA ACAAAAAATT GTTAATATAC
421 CTCTATACTT TAACGTCAAG GAGAAAAAAC CCCGGATCGG ACTACTAGCA GCTGTAATAC
481 GACTCACTAT AGGGAATATT AAGCTTGGTA CCATGAGATT TCCTTCAATT TTTACTGCAG
541 TTTTATTCGC AGCATCCTCC GCATTAGCTG CTCCAGTCAA CACTACAACA GAAGATGAAA
601 CGGCACAAAT TCCGGCTGAA GCTGTCATCG GTTACTCAGA TTTAGAAGGG GATTTCGATG
661 TTGCTGTTTT GCCATTTTCC AACAGCACAA ATAACGGGTT ATTGTTTATA AATACTACTA
721 TTGCCAGCAT TGCTGCTAAA GAAGAAGGGG TATCTCTCGA GAAAAGAGAG GCTGAAGCTT
781 ACGTAGAATT CCCTAGGGCG GCCGCGAATT CTAGAGGGCC CTTCGAAGGT AAGCCTATCC
841 CTAACCCTCT CCTCGGTCTC GATTCTACGC GTACCGGTCA TCATCACCAT CACCATTGAG
901 TTTAAACCCG CTGATCCTAG AGGGCCGCAT CATGTAATTA GTTATGTCAC GCTTACATTC
961 ACGCCCTCCC CCCACATCCG CTCTAACCGA AAAGGAAGGA GTTAGACAAC CTGAAGTCTA
1021 GGTCCCTATT TATTTTTTTA TAGTTATGTT AGTATTAAGA ACGTTATTTA TATTTCAAAT
1081 TTTTCTTTTT TTTCTGTACA GACGCGTGTA CGCATGTAAC ATTATACTGA AAACCTTGCT
1141 TGAGAAGGTT TTGGGACGCT CGAAGGCTTT AATTTGCAAG CTGCGGCCCT GCATTAATGA
1201 ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC
1261 ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG
1321 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC
1381 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC
1441 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA
1501 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC
1561 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT
1621 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG
1681 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC
1741 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA
1801 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT
1861 AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT
1921 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG
1981 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG
2041 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
2101 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
2161 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
2221 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
2281 CGGGAGCGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG
2341 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
2401 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
2461 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GGCATTGCTA CAGGCATCGT GGTGTCACTC
2521 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
2581 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
2641 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
2701 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
2761 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TAGTGTATCA
2821 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
2881 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
2941 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
3001 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
3061 TGGGTAATAA CTGATATAAT TAAATTGAAG CTCTAATTTG TGAGTTTAGT ATACATGCAT
3121 TTACTTATAA TACAGTTTTT TAGTTTTGCT GGCCGCATCT TCTCAAATAT GCTTCCCAGC
3181 CTGCTTTTCT GTAACGTTCA CCCTCTACCT TAGCATCCCT TCCCTTTGCA AATAGTCCTC
3241 TTCCAACAAT AATAATGTCA GATCCTGTAG AGACCACATC ATCCACGGTT CTATACTGTT
3301 GACCCAATGC GTCTCCCTTG TCATCTAAAC CCACACCGGG TGTCATAATC AACCAATCGT
3361 AACCTTCATC TCTTCCACCC ATGTCTCTTT GAGCAATAAA GCCGATAACA AAATCTTTGT
3421 CGCTCTTCGC AATGTCAACA GTACCCTTAG TATATTCTCC AGTAGATAGG GAGCCCTTGC
3481 ATGACAATTC TGCTAACATC AAAAGGCCTC TAGGTTCCTT TGTTACTTCT TCTGCCGCCT
3541 GCTTCAAACC GCTAACAATA CCTGGGCCCA CCACACCGTG TGCATTCGTA ATGTCTGCCC
3601 ATTCTGCTAT TCTGTATACA CCCGCAGAGT ACTGCAATTT GACTGTATTA CCAATGTCAG
3661 CAAATTTTCT GTCTTCGAAG AGTAAAAAAT TGTACTTGGC GGATAATGCC TTTAGCGGCT
3721 TAACTGTGCC CTCCATGGAA AAATCAGTCA AGATATCCAC ATGTGTTTTT AGTAAACAAA
3781 TTTTGGGACC TAATGCTTCA ACTAACTCCA GTAATTCCTT GGTGGTACGA ACATCCAATG
3841 AAGCACACAA GTTTGTTTGC TTTTCGTGCA TGATATTAAA TAGCTTGGCA GCAACAGGAC
3901 TAGGATGAGT AGCAGCACGT TCCTTATATG TAGCTTTCGA CATGATTTAT CTTCGTTTCC
3961 TGCAGGTTTT TGTTCTGTGC AGTTGGGTTA AGAATACTGG GCAATTTCAT GTTTCTTCAA
4021 CACTACATAT GCGTATATAT ACCAATCTAA GTCTGTGCTC CTTCCTTCGT TCTTCCTTCT
4081 GTTCGGAGAT TACCGAATCA AAAAAATTTC AAAGAAACCG AAATCAAAAA AAAGAATAAA
4141 AAAAAAATGA TGAATTGAAT TGAAAAGCTA GCTTATCGAT GATAAGCTGT CAAAGATGAG
4201 AATTAATTCC ACGGACTATA GACTATACTA GATACTCCGT CTACTGTACG ATACACTTCC
4261 GCTCAGGTCC TTGTCCTTTA ACGAGGCCTT ACCACTCTTT TGTTACTCTA TTGATCCAGC
4321 TCAGCAAAGG CAGTGTGATC TAAGATTCTA TCTTCGCGAT GTAGTAAAAC TAGCTAGACC
4381 GAGAAAGAGA CTAGAAATGC AAAAGGCACT TCTACAATGG CTGCCATCAT TATTATCCGA
4441 TGTGACGCTG CAGCTTCTCA ATGATATTCG AATACGCTTT GAGGAGATAC AGCCTAATAT
4501 CCGACAAACT GTTTTACAGA TTTACGATCG TACTTGTTAC CCATCATTGA ATTTTGAACA
4561 TCCGAACCTG GGAGTTTTCC CTGAAACAGA TAGTATATTT GAACCTGTAT AATAATATAT
4621 AGTCTAGCGC TTTACGGAAG ACAATGTATG TATTTCGGTT CCTGGAGAAA CTATTGCATC
4681 TATTGCATAG GTAATCTTGC ACGTCGCATC CCCGGTTCAT TTTCTGCGTT TCCATCTTGC
4741 ACTTCAATAG CATATCTTTG TTAACGAAGC ATCTGTGCTT CATTTTGTAG AACAAAAATG
4801 CAACGCGAGA GCGCTAATTT TTCAAACAAA GAATCTGAGC TGCATTTTTA CAGAACAGAA
4861 ATGCAACGCG AAAGCGCTAT TTTACCAACG AAGAATCTGT GCTTCATTTT TGTAAAACAA
4921 AAATGCAACG CGACGAGAGC GCTAATTTTT CAAACAAAGA ATCTGAGCTG CATTTTTACA
4981 GAACAGAAAT GCAACGCGAG AGCGCTATTT TACCAACAAA GAATCTATAC TTCTTTTTTG
5041 TTCTACAAAA ATGCATCCCG AGAGCGCTAT TTTTCTAACA AAGCATCTTA GATTACTTTT
5101 TTTCTCCTTT GTGCGCTCTA TAATGCAGTC TCTTGATAAC TTTTTGCACT GTAGGTCCGT
5161 TAAGGTTAGA AGAAGGCTAC TTTGGTGTCT ATTTTCTCTT CCATAAAAAA AGCCTGACTC
5221 CACTTCCCGC GTTTACTGAT TACTAGCGAA GCTGCGGGTG CATTTTTTCA AGATAAAGGC
5281 ATCCCCGATT ATATTCTATA CCGATGTGGA TTGCGCATAC TTTGTGAACA GAAAGTGATA
5341 GCGTTGATGA TTCTTCATTG GTCAGAAAAT TATGAACGGT TTCTTCTATT TTGTCTCTAT
5401 ATACTACGTA TAGGAAATGT TTACATTTTC GTATTGTTTT CGATTCACTC TATGAATAGT
5461 TCTTACTACA ATTTTTTTGT CTAAAGAGTA ATACTAGAGA TAAACATAAA AAATGTAGAG
5521 GTCGAGTTTA GATGCAAGTT CAAGGAGCGA AAGGTGGATG GGTAGGTTAT ATAGGGATAT
5581 AGCACAGAGA TATATAGCAA AGAGATACTT TTGAGCAATG TTTGTGGAAG CGGTATTCGC
5641 AATGGGAAGC TCCACCCCGG TTGATAATCA GAAAAGCCCC AAAAACAGGA AGATTGTATA
5701 AGCAAATATT TAAATTGTAA ACGTTAATAT TTTGTTAAAA TTCGCGTTAA ATTTTTGTTA
5761 AATCAGCTCA TTTTTTAACG AATAGCCCGA AATCGGCAAA ATCCCTTATA AATCAAAAGA
5821 ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTCCAAC AAGAGTCCAC TATTAAAGAA
5881 CGTGGACTCC AACGTCAAAG GGCGAAAAAG GGTCTATCAG GGCGATGGCC CACTACGTGA
5941 ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT AAAGCAGTAA ATCGGAAGGG
6001 TAAACGGATG CCCCCATTTA GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA
6061 AGGGAAGAAA GCGAAAGGAG CGGGGGCTAG GGCGGTGGGA AGTGTAGGGG TCACGCTGGG
6121 CGTAACCACC ACACCCGCCG CGCTTAATGG GGCGCTACAG GGCGCGTGGG GATGATCCAC
6181 TAGT
