| pCMV-VSV-G 逆转录病毒包装载体；信封载体 需配合pUMVC | ||||||||||||||||||||||||||||
| 发布时间:2018-05-28 12:11:00 | 浏览次数： | ||||||||||||||||||||||||||||
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测序及更多信息：https://pan.baidu.com/s/11a0yvD0nXUgqEJxIlwiKjQ?pwd=acej 采购地址：淘宝店铺地址
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出品公司: |
(Plasmid #8454) |
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载体名称: |
pCMV-VSV-G |
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质粒类型: |
逆转录病毒包装载体；包膜蛋白质粒 |
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高拷贝/低拷贝: |
高拷贝 |
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启动子: |
CMV |
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克隆方法: |
多克隆位点，限制性内切酶 |
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载体大小: |
6363 |
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5' 测序引物及序列: |
CMV-F: CGCAAATGGGCGGTAGGCGTG |
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3' 测序引物及序列: |
-- |
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载体抗性: |
氨苄青霉素 |
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筛选标记: |
-- |
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稳定性: |
稳表达 |
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组成型: |
组成型 |
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病毒/非病毒: |
逆病毒 |
全序列
1 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG
61 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG
121 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC
181 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA
241 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT
301 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC
361 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC
421 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC
481 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT
541 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG
601 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA
661 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA
721 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA
781 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG
841 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC
901 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG
961 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT
1021 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG
1081 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA
1141 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA
1201 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC
1261 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT
1321 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA
1381 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT
1441 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT
1501 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA
1561 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG
1621 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA
1681 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA
1741 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG
1801 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC
1861 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG
1921 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTAAATT GTAAGCGTTA ATATTTTGTT
1981 AAAATTCGCG TTAAATTTTT GTTAAATCAG CTCATTTTTT AACCAATAGG CCGAAATCGG
2041 CAAAATCCCT TATAAATCAA AAGAATAGAC CGAGATAGGG TTGAGTGTTG TTCCAGTTTG
2101 GAACAAGAGT CCACTATTAA AGAACGTGGA CTCCAACGTC AAAGGGCGAA AAACCGTCTA
2161 TCAGGGCGAT GGCCCACTAC GTGAACCATC ACCCTAATCA AGTTTTTTGG GGTCGAGGTG
2221 CCGTAAAGCA CTAAATCGGA ACCCTAAAGG GAGCCCCCGA TTTAGAGCTT GACGGGGAAA
2281 GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT
2341 GGCAAGTGTA GCGGTCACGC TGCGCGTAAC CACCACACCC GCCGCGCTTA ATGCGCCGCT
2401 ACAGGGCGCG TCCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG
2461 GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG
2521 GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG AGCGCGCGTA
2581 ATACGACTCA CTATAGGGCG AATTGGAGCT CCACCGCGGT GGCGGCCGCT CTAGAGAGCT
2641 TGGCCCATTG CATACGTTGT ATCCATATCA TAATATGTAC ATTTATATTG GCTCATGTCC
2701 AACATTACCG CCATGTTGAC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG
2761 GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC
2821 GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT
2881 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC
2941 CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA
3001 CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG
3061 GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAT
3121 CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT
3181 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTC
3241 CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGAGC
3301 TCGTTTAGTG AACCGTCAGA TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG
3361 AAGACACCGG GACCGATCCA GCCTCCGGTC GACCGATCCT GAGAACTTCA GGGTGAGTTT
3421 GGGGACCCTT GATTGTTCTT TCTTTTTCGC TATTGTAAAA TTCATGTTAT ATGGAGGGGG
3481 CAAAGTTTTC AGGGTGTTGT TTAGAATGGG AAGATGTCCC TTGTATCACC ATGGACCCTC
3541 ATGATAATTT TGTTTCTTTC ACTTTCTACT CTGTTGACAA CCATTGTCTC CTCTTATTTT
3601 CTTTTCATTT TCTGTAACTT TTTCGTTAAA CTTTAGCTTG CATTTGTAAC GAATTTTTAA
3661 ATTCACTTTT GTTTATTTGT CAGATTGTAA GTACTTTCTC TAATCACTTT TTTTTCAAGG
3721 CAATCAGGGT ATATTATATT GTACTTCAGC ACAGTTTTAG AGAACAATTG TTATAATTAA
3781 ATGATAAGGT AGAATATTTC TGCATATAAA TTCTGGCTGG CGTGGAAATA TTCTTATTGG
3841 TAGAAACAAC TACACCCTGG TCATCATCCT GCCTTTCTCT TTATGGTTAC AATGATATAC
3901 ACTGTTTGAG ATGAGGATAA AATACTCTGA GTCCAAACCG GGCCCCTCTG CTAACCATGT
3961 TCATGCCTTC TTCTCTTTCC TACAGCTCCT GGGCAACGTG CTGGTTGTTG TGCTGTCTCA
4021 TCATTTTGGC AAAGAATTCC TCGACGGATC CCTCGAGGAA TTCTGACACT ATGAAGTGCC
4081 TTTTGTACTT AGCCTTTTTA TTCATTGGGG TGAATTGCAA GTTCACCATA GTTTTTCCAC
4141 ACAACCAAAA AGGAAACTGG AAAAATGTTC CTTCTAATTA CCATTATTGC CCGTCAAGCT
4201 CAGATTTAAA TTGGCATAAT GACTTAATAG GCACAGCCTT ACAAGTCAAA ATGCCCAAGA
4261 GTCACAAGGC TATTCAAGCA GACGGTTGGA TGTGTCATGC TTCCAAATGG GTCACTACTT
4321 GTGATTTCCG CTGGTATGGA CCGAAGTATA TAACACATTC CATCCGATCC TTCACTCCAT
4381 CTGTAGAACA ATGCAAGGAA AGCATTGAAC AAACGAAACA AGGAACTTGG CTGAATCCAG
4441 GCTTCCCTCC TCAAAGTTGT GGATATGCAA CTGTGACGGA TGCCGAAGCA GTGATTGTCC
4501 AGGTGACTCC TCACCATGTG CTGGTTGATG AATACACAGG AGAATGGGTT GATTCACAGT
4561 TCATCAACGG AAAATGCAGC AATTACATAT GCCCCACTGT CCATAACTCT ACAACCTGGC
4621 ATTCTGACTA TAAGGTCAAA GGGCTATGTG ATTCTAACCT CATTTCCATG GACATCACCT
4681 TCTTCTCAGA GGACGGAGAG CTATCATCCC TGGGAAAGGA GGGCACAGGG TTCAGAAGTA
4741 ACTACTTTGC TTATGAAACT GGAGGCAAGG CCTGCAAAAT GCAATACTGC AAGCATTGGG
4801 GAGTCAGACT CCCATCAGGT GTCTGGTTCG AGATGGCTGA TAAGGATCTC TTTGCTGCAG
4861 CCAGATTCCC TGAATGCCCA GAAGGGTCAA GTATCTCTGC TCCATCTCAG ACCTCAGTGG
4921 ATGTAAGTCT AATTCAGGAC GTTGAGAGGA TCTTGGATTA TTCCCTCTGC CAAGAAACCT
4981 GGAGCAAAAT CAGAGCGGGT CTTCCAATCT CTCCAGTGGA TCTCAGCTAT CTTGCTCCTA
5041 AAAACCCAGG AACCGGTCCT GCTTTCACCA TAATCAATGG TACCCTAAAA TACTTTGAGA
5101 CCAGATACAT CAGAGTCGAT ATTGCTGCTC CAATCCTCTC AAGAATGGTC GGAATGATCA
5161 GTGGAACTAC CACAGAAAGG GAACTGTGGG ATGACTGGGC ACCATATGAA GACGTGGAAA
5221 TTGGACCCAA TGGAGTTCTG AGGACCAGTT CAGGATATAA GTTTCCTTTA TACATGATTG
5281 GACATGGTAT GTTGGACTCC GATCTTCATC TTAGCTCAAA GGCTCAGGTG TTCGAACATC
5341 CTCACATTCA AGACGCTGCT TCGCAACTTC CTGATGATGA GAGTTTATTT TTTGGTGATA
5401 CTGGGCTATC CAAAAATCCA ATCGAGCTTG TAGAAGGTTG GTTCAGTAGT TGGAAAAGCT
5461 CTATTGCCTC TTTTTTCTTT ATCATAGGGT TAATCATTGG ACTATTCTTG GTTCTCCGAG
5521 TTGGTATCCA TCTTTGCATT AAATTAAAGC ACACCAAGAA AAGACAGATT TATACAGACA
5581 TAGAGATGAA CCGACTTGGA AAGTAACTCA AATCCTGCAC AACAGATTCT TCATGTTTGG
5641 ACCAAATCAA CTTGTGATAC CATGCTCAAA GAGGCCTCAA TTATATTTGA GTTTTTAATT
5701 TTTATGAAAA AAAAAAAAAA AAACGGAATT CCTCGAGGGA TCCGTCGAGG AATTCACTCC
5761 TCAGGTGCAG GCTGCCTATC AGAAGGTGGT GGCTGGTGTG GCCAATGCCC TGGCTCACAA
5821 ATACCACTGA GATCTTTTTC CCTCTGCCAA AAATTATGGG GACATCATGA AGCCCCTTGA
5881 GCATCTGACT TCTGGCTAAT AAAGGAAATT TATTTTCATT GCAATAGTGT GTTGGAATTT
5941 TTTGTGTCTC TCACTCGGAA GGACATATGG GAGGGCAAAT CATTTAAAAC ATCAGAATGA
6001 GTATTTGGTT TAGAGTTTGG CAACATATGC CCATATGCTG GCTGCCATGA ACAAAGGTTG
6061 GCTATAAAGA GGTCATCAGT ATATGAAACA GCCCCCTGCT GTCCATTCCT TATTCCATAG
6121 AAAAGCCTTG ACTTGAGGTT AGATTTTTTT TATATTTTGT TTTGTGTTAT TTTTTTCTTT
6181 AACATCCCTA AAATTTTCCT TACATGTTTT ACTAGCCAGA TTTTTCCTCC TCTCCTGACT
6241 ACTCCCAGTC ATAGCTGTCC CTCTTCTCTT ATGGAGATCC CTCGACGGAT CGGCCGCAAT
6301 TCGTAATCAT GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA
6361 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA
6421 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC
6481 ATTAATGAAT CGGCCAACGC GCGGGGA
