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腺病毒质粒

pShuttle-IRES-hrGFP-1 腺病毒表达载体穿梭质粒 3×Flag 绿色荧光蛋白
发布时间:2018-05-28 16:48:49 | 浏览次数:


测序及更多信息:https://pan.baidu.com/s/1wvCgCS6yWmB4ej1uNJEZVw 密码:wigx

采购地址:淘宝店铺地址  


载体基本信息

出品公司:

Agilent

载体名称:

pShuttle-IRES-hrGFP-1

质粒类型:

腺病毒表达载体,哺乳动物细胞表达载体

高拷贝/低拷贝:

低拷贝

启动子:

CMV

克隆方法:

多克隆位点,限制性内切酶

载体大小:

8.9 kb

5' 测序引物及序列:

pShuttle-IRES-hrGFP-1-F: CTCACGGGGATTTCCAAGTC

3' 测序引物及序列:

pShuttle-IRES-hrGFP-1-R: ATGCAGTCGTCGAGGAATTG

载体标签:

--

载体抗性:

卡那

备注:

载体内包含有IRES序列和hrGFP序列。

稳定性:

/

组成型:

--

病毒/非病毒:

腺病毒


全序列

1        CATCATCAAT AATATACCTT ATTTTGGATT GAAGCCAATA TGATAATGAG GGGGTGGAGT
61       TTGTGACGTG GCGCGGGGCG TGGGAACGGG GCGGGTGACG TAGTAGTGTG GCGGAAGTGT
121      GATGTTGCAA GTGTGGCGGA ACACATGTAA GCGACGGATG TGGCAAAAGT GACGTTTTTG
181      GTGTGCGCCG GTGTACACAG GAAGTGACAA TTTTCGCGCG GTTTTAGGCG GATGTTGTAG
241      TAAATTTGGG CGTAACCGAG TAAGATTTGG CCATTTTCGC GGGAAAACTG AATAAGAGGA
301      AGTGAAATCT GAATAATTTT GTGTTACTCA TAGCGCGTAA TACTGTAATA GTAATCAATT
361      ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT
421      GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT
481      CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA
541      ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC
601      AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTATG GGACTTTCCT
661      ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG
721      TACATCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT
781      GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC
841      AACTCCGCCC CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
901      AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGAGATCTG CGGCCGCAGT ACTGCTAGCA
961      CTAGTGATAT CCGATCGGTC GACGCCCGGG CCTCGAGGAC TACAAGGATG ACGATGACAA
1021     GGATTACAAA GACGACGATG ATAAGGACTA TAAGGATGAT GACGACAAAT AATAGCAATT
1081     CCTCGACGAC TGCATAGGGT TACCCCCCTC TCCCTCCCCC CCCCCTAACG TTACTGGCCG
1141     AAGCCGCTTG GAATAAGGCC GGTGTGCGTT TGTCTATATG TTATTTTCCA CCATATTGCC
1201     GTCTTTTGGC AATGTGAGGG CCCGGAAACC TGGCCCTGTC TTCTTGACGA GCATTCCTAG
1261     GGGTCTTTCC CCTCTCGCCA AAGGAATGCA AGGTCTGTTG AATGTCGTGA AGGAAGCAGT
1321     TCCTCTGGAA GCTTCTTGAA GACAAACAAC GTCTGTAGCG ACCCTTTGCA GGCAGCGGAA
1381     CCCCCCACCT GGCGACAGGT GCCTCTGCGG CCAAAAGCCA CGTGTATAAG ATACACCTGC
1441     AAAGGCGGCA CAACCCCAGT GCCACGTTGT GAGTTGGATA GTTGTGGAAA GAGTCAAATG
1501     GCTCTCCTCA AGCGTATTCA ACAAGGGGCT GAAGGATGCC CAGAAGGTAC CCCATTGTAT
1561     GGGATCTGAT CTGGGGCCTC GGTGCACATG CTTTACATGT GTTTAGTCGA GGTTAAAAAA
1621     CGTCTAGGCC CCCCGAACCA CGGGGACGTG GTTTTCCTTT GAAAAACACG ATGATAATGG
1681     CCACAACCAT GGTGAGCAAG CAGATCCTGA AGAACACCGG CCTGCAGGAG ATCATGAGCT
1741     TCAAGGTGAA CCTGGAGGGC GTGGTGAACA ACCACGTGTT CACCATGGAG GGCTGCGGCA
1801     AGGGCAACAT CCTGTTCGGC AACCAGCTGG TGCAGATCCG CGTGACCAAG GGCGCCCCCC
1861     TGCCCTTCGC CTTCGACATC CTGAGCCCCG CCTTCCAGTA CGGCAACCGC ACCTTCACCA
1921     AGTACCCCGA GGACATCAGC GACTTCTTCA TCCAGAGCTT CCCCGCCGGC TTCGTGTACG
1981     AGCGCACCCT GCGCTACGAG GACGGCGGCC TGGTGGAGAT CCGCAGCGAC ATCAACCTGA
2041     TCGAGGAGAT GTTCGTGTAC CGCGTGGAGT ACAAGGGCCG CAACTTCCCC AACGACGGCC
2101     CCGTGATGAA GAAGACCATC ACCGGCCTGC AGCCCAGCTT CGAGGTGGTG TACATGAACG
2161     ACGGCGTGCT GGTGGGCCAG GTGATCCTGG TGTACCGCCT GAACAGCGGC AAGTTCTACA
2221     GCTGCCACAT GCGCACCCTG ATGAAGAGCA AGGGCGTGGT GAAGGACTTC CCCGAGTACC
2281     ACTTCATCCA GCACCGCCTG GAGAAGACCT ACGTGGAGGA CGGCGGCTTC GTGGAGCAGC
2341     ACGAGACCGC CATCGCCCAG CTGACCAGCC TGGGCAAGCC CCTGGGCAGC CTGCACGAGT
2401     GGGTGTAATA GGCGGCCATC CGATCCACCG GATCTAGATA ACTGATCATA ATCAGCCATA
2461     CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA
2521     AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA
2581     AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT
2641     GTGGTTTGTC CAAACTCATC AATGTATCTT AACGCGGATC TGGGCGTGGT TAAGGGTGGG
2701     AAAGAATATA TAAGGTGGGG GTCTTATGTA GTTTTGTATC TGTTTTGCAG CAGCCGCCGC
2761     CGCCATGAGC ACCAACTCGT TTGATGGAAG CATTGTGAGC TCATATTTGA CAACGCGCAT
2821     GCCCCCATGG GCCGGGGTGC GTCAGAATGT GATGGGCTCC AGCATTGATG GTCGCCCCGT
2881     CCTGCCCGCA AACTCTACTA CCTTGACCTA CGAGACCGTG TCTGGAACGC CGTTGGAGAC
2941     TGCAGCCTCC GCCGCCGCTT CAGCCGCTGC AGCCACCGCC CGCGGGATTG TGACTGACTT
3001     TGCTTTCCTG AGCCCGCTTG CAAGCAGTGC AGCTTCCCGT TCATCCGCCC GCGATGACAA
3061     GTTGACGGCT CTTTTGGCAC AATTGGATTC TTTGACCCGG GAACTTAATG TCGTTTCTCA
3121     GCAGCTGTTG GATCTGCGCC AGCAGGTTTC TGCCCTGAAG GCTTCCTCCC CTCCCAATGC
3181     GGTTTAAAAC ATAAATAAAA AACCAGACTC TGTTTGGATT TGGATCAAGC AAGTGTCTTG
3241     CTGTCTTTAT TTAGGGGTTT TGCGCGCGCG GTAGGCCCGG GACCAGCGGT CTCGGTCGTT
3301     GAGGGTCCTG TGTATTTTTT CCAGGACGTG GTAAAGGTGA CTCTGGATGT TCAGATACAT
3361     GGGCATAAGC CCGTCTCTGG GGTGGAGGTA GCACCACTGC AGAGCTTCAT GCTGCGGGGT
3421     GGTGTTGTAG ATGATCCAGT CGTAGCAGGA GCGCTGGGCG TGGTGCCTAA AAATGTCTTT
3481     CAGTAGCAAG CTGATTGCCA GGGGCAGGCC CTTGGTGTAA GTGTTTACAA AGCGGTTAAG
3541     CTGGGATGGG TGCATACGTG GGGATATGAG ATGCATCTTG GACTGTATTT TTAGGTTGGC
3601     TATGTTCCCA GCCATATCCC TCCGGGGATT CATGTTGTGC AGAACCACCA GCACAGTGTA
3661     TCCGGTGCAC TTGGGAAATT TGTCATGTAG CTTAGAAGGA AATGCGTGGA AGAACTTGGA
3721     GACGCCCTTG TGACCTCCAA GATTTTCCAT GCATTCGTCC ATAATGATGG CAATGGGCCC
3781     ACGGGCGGCG GCCTGGGCGA AGATATTTCT GGGATCACTA ACGTCATAGT TGTGTTCCAG
3841     GATGAGATCG TCATAGGCCA TTTTTACAAA GCGCGGGCGG AGGGTGCCAG ACTGCGGTAT
3901     AATGGTTCCA TCCGGCCCAG GGGCGTAGTT ACCCTCACAG ATTTGCATTT CCCACGCTTT
3961     GAGTTCAGAT GGGGGGATCA TGTCTACCTG CGGGGCGATG AAGAAAACGG TTTCCGGGGT
4021     AGGGGAGATC AGCTGGGAAG AAAGCAGGTT CCTGAGCAGC TGCGACTTAC CGCAGCCGGT
4081     GGGCCCGTAA ATCACACCTA TTACCGGCTG CAACTGGTAG TTAAGAGAGC TGCAGCTGCC
4141     GTCATCCCTG AGCAGGGGGG CCACTTCGTT AAGCATGTCC CTGACTCGCA TGTTTTCCCT
4201     GACCAAATCC GCCAGAAGGC GCTCGCCGCC CAGCGATAGC AGTTCTTGCA AGGAAGCAAA
4261     GTTTTTCAAC GGTTTGAGAC CGTCCGCCGT AGGCATGCTT TTGAGCGTTT GACCAAGCAG
4321     TTCCAGGCGG TCCCACAGCT CGGTCACCTG CTCTACGGCA TCTCGATCCA GCATATCTCC
4381     TCGTTTCGCG GGTTGGGGCG GCTTTCGCTG TACGGCAGTA GTCGGTGCTC GTCCAGACGG
4441     GCCAGGGTCA TGTCTTTCCA CGGGCGCAGG GTCCTCGTCA GCGTAGTCTG GGTCACGGTG
4501     AAGGGGTGCG CTCCGGGCTG CGCGCTGGCC AGGGTGCGCT TGAGGCTGGT CCTGCTGGTG
4561     CTGAAGCGCT GCCGGTCTTC GCCCTGCGCG TCGGCCAGGT AGCATTTGAC CATGGTGTCA
4621     TAGTCCAGCC CCTCCGCGGC GTGGCCCTTG GCGCGCAGCT TGCCCTTGGA GGAGGCGCCG
4681     CACGAGGGGC AGTGCAGACT TTTGAGGGCG TAGAGCTTGG GCGCGAGAAA TACCGATTCC
4741     GGGGAGTAGG CATCCGCGCC GCAGGCCCCG CAGACGGTCT CGCATTCCAC GAGCCAGGTG
4801     AGCTCTGGCC GTTCGGGGTC AAAAACCAGG TTTCCCCCAT GCTTTTTGAT GCGTTTCTTA
4861     CCTCTGGTTT CCATGAGCCG GTGTCCACGC TCGGTGACGA AAAGGCTGTC CGTGTCCCCG
4921     TATACAGACT TGAGAGGGAG TTTAAACGAA TTCAATAGCT TGTTGCATGG GCGGCGATAT
4981     AAAATGCAAG GTGCTGCTCA AAAAATCAGG CAAAGCCTCG CGCAAAAAAG AAAGCACATC
5041     GTAGTCATGC TCATGCAGAT AAAGGCAGGT AAGCTCCGGA ACCACCACAG AAAAAGACAC
5101     CATTTTTCTC TCAAACATGT CTGCGGGTTT CTGCATAAAC ACAAAATAAA ATAACAAAAA
5161     AACATTTAAA CATTAGAAGC CTGTCTTACA ACAGGAAAAA CAACCCTTAT AAGCATAAGA
5221     CGGACTACGG CCATGCCGGC GTGACCGTAA AAAAACTGGT CACCGTGATT AAAAAGCACC
5281     ACCGACAGCT CCTCGGTCAT GTCCGGAGTC ATAATGTAAG ACTCGGTAAA CACATCAGGT
5341     TGATTCACAT CGGTCAGTGC TAAAAAGCGA CCGAAATAGC CCGGGGGAAT ACATACCCGC
5401     AGGCGTAGAG ACAACATTAC AGCCCCCATA GGAGGTATAA CAAAATTAAT AGGAGAGAAA
5461     AACACATAAA CACCTGAAAA ACCCTCCTGC CTAGGCAAAA TAGCACCCTC CCGCTCCAGA
5521     ACAACATACA GCGCTTCCAC AGCGGCAGCC ATAACAGTCA GCCTTACCAG TAAAAAAGAA
5581     AACCTATTAA AAAAACACCA CTCGACACGG CACCAGCTCA ATCAGTCACA GTGTAAAAAA
5641     GGGCCAAGTG CAGAGCGAGT ATATATAGGA CTAAAAAATG ACGTAACGGT TAAAGTCCAC
5701     AAAAAACACC CAGAAAACCG CACGCGAACC TACGCCCAGA AACGAAAGCC AAAAAACCCA
5761     CAACTTCCTC AAATCGTCAC TTCCGTTTTC CCACGTTACG TCACTTCCCA TTTTAAGAAA
5821     ACTACAATTC CCAACACATA CAAGTTACTC CGCCCTAAAA CCTACGTCAC CCGCCCCGTT
5881     CCCACGCCCC GCGCCACGTC ACAAACTCCA CCCCCTCATT ATCATATTGG CTTCAATCCA
5941     AAATAAGGTA TATTATTGAT GATGTTAATT AACATGCATG GATCCATATG CGGTGTGAAA
6001     TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG CTCTTCCGCT TCCTCGCTCA
6061     CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG
6121     TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC
6181     AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC
6241     CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC
6301     TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC
6361     TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA
6421     GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC
6481     ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA
6541     ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG
6601     CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA
6661     GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG
6721     GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC
6781     AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT
6841     CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA
6901     GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT
6961     ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA
7021     TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC
7081     GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG
7141     CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG
7201     CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT
7261     CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTGC AGCCATGAGA TTATCAAAAA
7321     GGATCTTCAC CTAGATCCTT TTCACGTAGA AAGCCAGTCC GCAGAAACGG TGCTGACCCC
7381     GGATGAATGT CAGCTACTGG GCTATCTGGA CAAGGGAAAA CGCAAGCGCA AAGAGAAAGC
7441     AGGTAGCTTG CAGTGGGCTT ACATGGCGAT AGCTAGACTG GGCGGTTTTA TGGACAGCAA
7501     GCGAACCGGA ATTGCCAGCT GGGGCGCCCT CTGGTAAGGT TGGGAAGCCC TGCAAAGTAA
7561     ACTGGATGGC TTTCTTGCCG CCAAGGATCT GATGGCGCAG GGGATCAAGC TCTGATCAAG
7621     AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG
7681     CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG
7741     ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC
7801     TGTCCGGTGC CCTGAATGAA CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA
7861     CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC
7921     TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG
7981     TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT
8041     TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG
8101     TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA
8161     GGCTCAAGGC GAGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT
8221     TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG
8281     GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG
8341     GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC
8401     GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAAT TTTGTTAAAA TTTTTGTTAA
8461     ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCACCA TCCCTTATAA ATCAAAAGAA
8521     TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC
8581     GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA
8641     CCATCACCCT AATCAAGTTT TTTGTGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT
8701     AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA
8761     GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC
8821     GTAACCACCA CACCCGCGCG CTTAATGCGC CGCTACAGGG CGCGTCCATT CGCCATTCAG
8881     GATCGAATTA ATTCTTAATT AA




 
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