测序及更多信息：https://pan.baidu.com/s/1RqX_08qoD2DNNR4n4LzmwQ 密码：ppg5
采购地址：淘宝店铺地址
|
启动子:
|
CaMV 35S,T7
|
|
复制子:
|
pSa ori，ori
|
|
质粒分类:
|
植物表达载体,启动子报告载体
|
|
质粒大小:
|
6383bp
|
|
原核抗性:
|
Kan
|
|
克隆菌株:
|
DH5α
|
|
培养条件:
|
37℃，有氧 LB
|
|
表达宿主:
|
植物细胞
|
|
5'测序引物:
|
35S:GACGCACAATCCCACTATCC
|
|
3'测序引物:
|
根据序列设计引物
|
|
内参基因:
|
Rluc
|
|
报告基因:
|
Luc
|
|
搭配质粒：
|
pGreenII 62-SK
|
全序列
1 TTTTTATCCC CGGAAGCCTG TGGATAGAGG GTAGTTATCC ACGTGAAACC GCTAATGCCC
61 CGCAAAGCCT TGATTCACGG GGCTTTCCGG CCCGCTCCAA AAACTATCCA CGTGAAATCG
121 CTAATCAGGG TACGTGAAAT CGCTAATCGG AGTACGTGAA ATCGCTAATA AGGTCACGTG
181 AAATCGCTAA TCAAAAAGGC ACGTGAGAAC GCTAATAGCC CTTTCAGATC AACAGCTTGC
241 AAACACCCCT CGCTCCGGCA AGTAGTTACA GCAAGTAGTA TGTTCAATTA GCTTTTCAAT
301 TATGAATATA TATATCAATT ATTGGTCGCC CTTGGCTTGT GGACAATGCG CTACGCGCAC
361 CGGCTCCGCC CGTGGACAAC CGCAAGCGGT TGCCCACCGT CGAGCGCCAG CGCCTTTGCC
421 CACAACCCGG CGGCCGGCCG CAACAGATCG TTTTATAAAT TTTTTTTTTT GAAAAAGAAA
481 AAGCCCGAAA GGCGGCAACC TCTCGGGCTT CTGGATTTCC GATCCCCGGA ATTAGAGATC
541 TTGGCAGGAT ATATTGTGGT GTAACGTTAT CGTACCCCTA CTCCAAAAAT GTCAAAGATA
601 CAGTCTCAGA AGACCAAAGG GCTATTGAGA CTTTTCAACA AAGGGTAATT TCGGGAAACC
661 TCCTCGGATT CCATTGCCCA GCTATCTGTC ACTTCATCGA AAGGACAGTA GAAAAGGAAG
721 GTGGCTCCTA CAAATGCCAT CATTGCGATA AAGGAAAGGC TATCATTCAA GATGCCTCTG
781 CCGACAGTGG TCCCAAAGAT GGACCCCCAC CCACGAGGAG CATCGTGGAA AAAGAAGACG
841 TTCCAACCAC GTCTTCAAAG CAAGTGGATT GATGTGACAT CTCCACTGAC GTAAGGGATG
901 ACGCACAATC CCACTATCCT TCGCAAGACC CTTCCTCTAT ATAAGGAAGT TCATTTCATT
961 TGGAGAGGAC AGCCCACCAC CATGACTTCG AAAGTTTATG ATCCAGAACA AAGGAAACGG
1021 ATGATAACTG GTCCGCAGTG GTGGGCCAGA TGTAAACAAA TGAATGTTCT TGATTCATTT
1081 ATTAATTATT ATGATTCAGA AAAACATGCA GAAAATGCTG TTATTTTTTT ACATGGTAAC
1141 GCGGCCTCTT CTTATTTATG GCGACATGTT GTGCCACATA TTGAGCCAGT AGCGCGGTGT
1201 ATTATACCAG ACCTTATTGG TATGGGCAAA TCAGGCAAAT CTGGTAATGG TTCTTATAGG
1261 TTACTTGATC ATTACAAATA TCTTACTGCA TGGTTTGAAC TTCTTAATTT ACCAAAGAAG
1321 ATCATTTTTG TCGGCCATGA TTGGGGTGCT TGTTTGGCAT TTCATTATAG CTATGAGCAT
1381 CAAGATAAGA TCAAAGCAAT AGTTCACGCT GAAAGTGTAG TAGATGTGAT TGAATCATGG
1441 GATGAATGGC CTGATATTGA AGAAGATATT GCGTTGATCA AATCTGAAGA AGGAGAAAAA
1501 ATGGTTTTGG AGAATAACTT CTTCGTGGAA ACCATGTTGC CATCAAAAAT CATGAGAAAG
1561 TTAGAACCAG AAGAATTTGC AGCATATCTT GAACCATTCA AAGAGAAAGG TGAAGTTCGT
1621 CGTCCAACAT TATCATGGCC TCGTGAAATC CCGTTAGTAA AAGGTGGTAA ACCTGACGTT
1681 GTACAAATTG TTAGGAATTA TAATGCTTAT CTACGTGCAA GTGATGATTT ACCAAAAATG
1741 TTTATTGAAT CGGACCCAGG ATTCTTTTCC AATGCTATTG TTGAAGGTGC CAAGAAGTTT
1801 CCTAATACTG AATTTGTCAA AGTAAAAGGT CTTCATTTTT CGCAAGAAGA TGCACCTGAT
1861 GAAATGGGAA AATATATCAA ATCGTTCGTT GAGCGAGTTC TCAAAAATGA ACAATAATTC
1921 TAGGGTACGC TGAAATCACC AGTCTCTCTC TACAAATCTA TCTCTCTCTA TTTTCTCCAT
1981 AAATAATGTG TGAGTAGTTT CCCGATAAGG GAAATTAGGG TTCTTATAGG GTTTCGCTCA
2041 TGTGTTGAGC ATATAAGAAA CCCTTAGTAT GTATTTGTAT TTGTAAAATA CTTCTATCAA
2101 TAAAATTTCT AATTCCTAAA ACCAAAATCC AGTACTAAAA TCCAGATCGA TAACATTAAC
2161 GCTTACAATT TCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG
2221 GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG
2281 GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA GCGCGCGTAA
2341 TACGACTCAC TATAGGGCGA ATTGGGTACC GGGCCCCCCC TCGAGGTCGA CGGTATCGAT
2401 AAGCTTGATA TCGAATTCCT GCAGCCCGGG GGATCCACTA GTTCTAGAGC GGCCGCCACC
2461 GCGGTGGAGA TCGAATTCCA TGGAAGACGC CAAAAACATA AAGAAAGGCC CGGCGCCATT
2521 CTATCCGCTG GAAGATGGAA CCGCTGGAGA GCAACTGCAT AAGGCTATGA AGAGATACGC
2581 CCTGGTTCCT GGAACAATTG CTTTTACAGA TGCACATATC GAGGTGGACA TCACTTACGC
2641 TGAGTACTTC GAAATGTCCG TTCGGTTGGC AGAAGCTATG AAACGATATG GGCTGAATAC
2701 AAATCACAGA ATCGTCGTAT GCAGTGAAAA CTCTCTTCAA TTCTTTATGC CGGTGTTGGG
2761 CGCGTTATTT ATCGGAGTTG CAGTTGCGCC CGCGAACGAC ATTTATAATG AACGTGAATT
2821 GCTCAACAGT ATGGGCATTT CGCAGCCTAC CGTGGTGTTC GTTTCCAAAA AGGGGTTGCA
2881 AAAAATTTTG AACGTGCAAA AAAAGCTCCC AATCATCCAA AAAATTATTA TCATGGATTC
2941 TAAAACGGAT TACCAGGGAT TTCAGTCGAT GTACACGTTC GTCACATCTC ATCTACCTCC
3001 CGGTTTTAAT GAATACGATT TTGTGCCAGA GTCCTTCGAT AGGGACAAGA CAATTGCACT
3061 GATCATGAAC TCCTCTGGAT CTACTGGTCT GCCTAAAGGT GTCGCTCTGC CTCATAGAAC
3121 TGCCTGCGTG AGATTCTCGC ATGCCAGAGA TCCTATTTTT GGCAATCAAA TCATTCCGGA
3181 TACTGCGATT TTAAGTGTTG TTCCATTCCA TCACGGTTTT GGAATGTTTA CTACACTCGG
3241 ATATTTGATA TGTGGATTTC GAGTCGTCTT AATGTATAGA TTTGAAGAAG AGCTGTTTCT
3301 GAGGAGCCTT CAGGATTACA AGATTCAAAG TGCGCTGCTG GTGCCAACCC TATTCTCCTT
3361 CTTCGCCAAA AGCACTCTGA TTGACAAATA CGATTTATCT AATTTACACG AAATTGCTTC
3421 TGGTGGCGCT CCCCTCTCTA AGGAAGTCGG GGAAGCGGTT GCCAAGAGGT TCCATCTGCC
3481 AGGTATCAGG CAAGGATATG GGCTCACTGA GACTACATCA GCTATTCTGA TTACACCCGA
3541 GGGGGATGAT AAACCGGGCG CGGTCGGTAA AGTTGTTCCA TTTTTTGAAG CGAAGGTTGT
3601 GGATCTGGAT ACCGGGAAAA CGCTGGGCGT TAATCAAAGA GGCGAACTGT GTGTGAGAGG
3661 TCCTATGATT ATGTCCGGTT ATGTAAACAA TCCGGAAGCG ACCAACGCCT TGATTGACAA
3721 GGATGGATGG CTACATTCTG GAGACATAGC TTACTGGGAC GAAGACGAAC ACTTCTTCAT
3781 CGTTGACCGC CTGAAGTCTC TGATTAAGTA CAAAGGCTAT CAGGTGGCTC CCGCTGAATT
3841 GGAATCCATC TTGCTCCAAC ACCCCAACAT CTTCGACGCA GGTGTCGCAG GTCTTCCCGA
3901 CGATGACGCC GGTGAACTTC CCGCCGCCGT TGTTGTTTTG GAGCACGGAA AGACGATGAC
3961 GGAAAAAGAG ATCGTGGATT ACGTCGCCAG TCAAGTAACA ACCGCGAAAA AGTTGCGCGG
4021 AGGAGTTGTG TTTGTGGACG AAGTACCGAA AGGTCTTACC GGAAAACTCG ACGCAAGAAA
4081 AATCAGAGAG ATCCTCATAA AGGCCAAGAA GGGCGGAAAG ATCGCCGTGT AATTCTAGAG
4141 AATTCGCTGA AATCACCAGT CTCTCTCTAC AAATCTATCT CTCTCTATTT TCTCCATAAA
4201 TAATGTGTGA GTAGTTTCCC GATAAGGGAA ATTAGGGTTC TTATAGGGTT TCGCTCATGT
4261 GTTGAGCATA TAAGAAACCC TTAGTATGTA TTTGTATTTG TAAAATACTT CTATCAATAA
4321 AATTTCTAAT TCCTAAAACC AAAATCCAGT ACTAAAATCC AGATCCACTA GCCTTGACAG
4381 GATATATTGG CGGGTAAACT AAGTCGCTGT ATGTGTTTGT TTGAGATCTC ATGTGAGCAA
4441 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC
4501 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA
4561 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC
4621 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT
4681 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT
4741 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG
4801 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA
4861 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT
4921 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAGAA
4981 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT
5041 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA
5101 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT
5161 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA
5221 GTATATATGT GTAACATTGG TCTAGTGATT AGAAAAACTC ATCGAGCATC AAATGAAACT
5281 GCAATTTATT CATATCAGGA TTATCAATAC CATATTTTTG AAAAAGCCGT TTCTGTAATG
5341 AAGGAGAAAA CTCACCGAGG CAGTTCCATA GGATGGCAAG ATCCTGGTAT CGGTCTGCGA
5401 TTCCGACTCG TCCAACATCA ATACAACCTA TTAATTTCCC CTCGTCAAAA ATAAGGTTAT
5461 CAAGTGAGAA ATCACCATGA GTGACGACTG AATCCGGTGA GAATGGCAAA AGTTTATGCA
5521 TTTCTTTCCA GACTTGTTCA ACAGGCCAGC CATTACGCTC GTCATCAAAA TCACTCGCAT
5581 CAACCAAACC GTTATTCATT CGTGATTGCG CCTGAGCGAG ACGAAATACG CGATCGCTGT
5641 TAAAAGGACA ATTACAAACA GGAATCGAAT GCAACCGGCG CAGGAACACT GCCAGCGCAT
5701 CAACAATATT TTCACCTGAA TCAGGATATT CTTCTAATAC CTGGAATGCT GTTTTCCCTG
5761 GGATCGCAGT GGTGAGTAAC CATGCATCAT CAGGAGTACG GATAAAATGC TTGATGGTCG
5821 GAAGAGGCAT AAATTCCGTC AGCCAGTTTA GTCTGACCAT CTCATCTGTA ACAACATTGG
5881 CAACGCTACC TTTGCCATGT TTCAGAAACA ACTCTGGCGC ATCGGGCTTC CCATACAATC
5941 GGTAGATTGT CGCACCTGAT TGCCCGACAT TATCGCGAGC CCATTTATAC CCATATAAAT
6001 CAGCATCCAT GTTGGAATTT AATCGCGGCC TTGAGCAAGA CGTTTCCCGT TGAATATGGC
6061 TCATAACACC CCTTGTATTA CTGTTTATGT AAGCAGACAG TTTTATTGTT CATGATGATA
6121 TATTTTTATC TTGTGCAATG TAACATCAGA GATTTTGAGA CACAACGTGG CTTTGTTGAA
6181 TAAATCGAAC TTTTGCTGAG TTGAAGGATC AGATCACGCA TCTTCCCGAC AACGCAGACC
6241 GTTCCGTGGC AAAGCAAAAG TTCAAAATCA CCAACTGGTC CACCTACAAC AAAGCTCTCA
6301 TCAACCGTGG CTCCCTCACT TTCTGGCTGG ATGATGGGGC GATTCAGGCG ATCCCCATCC
6361 AACAGCCCGC CGTCGAGCGG GCT
