测序及更多信息：
采购地址：淘宝店铺地址
我们的官网 http://hedgehogbio.comhttp://hedgehogbio.com/
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出品公司:
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海吉浩格HedgehogBio
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载体名称:
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pCMV-Myc-SP1
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质粒类型:
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哺乳动物细胞蛋白表达载体
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启动子:
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CMV
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表达水平:
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高
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克隆方法:
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多克隆位点，限制性内切酶
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载体大小:
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6131 bp
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5' 测序引物:
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CMV-F
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3' 测序引物:
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SV40pA-R
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插入基因:
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SP1( NM_138473.2)
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载体抗性:
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Ampicillin 氨苄
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筛选标记:
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--
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稳定性:
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瞬表达 Transient
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组成型:
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组成型 Constitutive
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病毒/非病毒:
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非病毒
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全序列
1 GAGTTCGAGC TTGCATGCCT GCAGGTCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC
61 TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG
121 CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG
181 GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA
241 TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC
301 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG
361 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG
421 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA
481 TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA
541 GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGACAC
601 CGGGACCGAT CCAGCCTCCG GACTCTAGAG GATCCGGTAC TAGAGGAACT GAAAAACCAG
661 AAAGTTAACT GGTAAGTTTA GTCTTTTTGT CTTTTATTTC AGGTCCCGGA TCCGGTGGTG
721 GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT ACTTCTAGGC CTGTACGGAA
781 GTGTTACTTC TGCTCTAAAA GCTGCGGAAT TGTACCCGCG GGCCCACCAT GGCATCAATG
841 CAGAAGCTGA TCTCAGAGGA GGACCTGCTT ATGGCCATGG AGGCCCGAAT TCGGATGAGC
901 GACCAAGATC ACTCCATGGA TGAAATGACA GCTGTGGTGA AAATTGAAAA AGGAGTTGGT
961 GGCAATAATG GGGGCAATGG TAATGGTGGT GGTGCCTTTT CACAGGCTCG AAGTAGCAGC
1021 ACAGGCAGTA GCAGCAGCAC TGGAGGAGGA GGGCAGGAGT CCCAGCCATC CCCTTTGGCT
1081 CTGCTGGCAG CAACTTGCAG CAGAATTGAG TCACCCAATG AGAACAGCAA CAACTCCCAG
1141 GGCCCGAGTC AGTCAGGGGG AACAGGTGAG CTTGACCTCA CAGCCACACA ACTTTCACAG
1201 GGTGCCAATG GCTGGCAGAT CATCTCTTCC TCCTCTGGGG CTACCCCTAC CTCAAAGGAA
1261 CAGAGTGGCA GCAGTACCAA TGGCAGCAAT GGCAGTGAGT CTTCCAAGAA TCGCACAGTC
1321 TCTGGTGGGC AGTATGTTGT GGCTGCCGCT CCCAACTTAC AGAACCAGCA AGTTCTGACA
1381 GGACTACCTG GAGTGATGCC TAATATTCAG TATCAAGTAA TCCCACAGTT CCAGACCGTT
1441 GATGGGCAAC AGCTGCAGTT TGCTGCCACT GGGGCCCAAG TGCAGCAGGA TGGTTCTGGT
1501 CAAATACAGA TCATACCAGG TGCAAACCAA CAGATTATCA CAAATCGAGG AAGTGGAGGC
1561 AACATCATTG CTGCTATGCC AAACCTACTC CAGCAGGCTG TCCCCCTCCA AGGCCTGGCT
1621 AATAATGTAC TCTCAGGACA GACTCAGTAT GTGACCAATG TACCAGTGGC CCTGAATGGG
1681 AACATCACCT TGCTACCTGT CAACAGCGTT TCTGCAGCTA CCTTGACTCC CAGCTCTCAG
1741 GCAGTCACGA TCAGCAGCTC TGGGTCCCAG GAGAGTGGCT CACAGCCTGT CACCTCAGGG
1801 ACTACCATCA GTTCTGCCAG CTTGGTATCA TCACAAGCCA GTTCCAGCTC CTTTTTCACC
1861 AATGCCAATA GCTACTCAAC TACTACTACC ACCAGCAACA TGGGAATTAT GAACTTTACT
1921 ACCAGTGGAT CATCAGGGAC CAACTCTCAA GGCCAGACAC CCCAGAGGGT CAGTGGGCTA
1981 CAGGGGTCTG ATGCTCTGAA CATCCAGCAA AACCAGACAT CTGGAGGCTC ATTGCAAGCA
2041 GGCCAGCAAA AAGAAGGAGA GCAAAACCAG CAGACACAGC AGCAACAAAT TCTTATCCAG
2101 CCTCAGCTAG TTCAAGGGGG ACAGGCCCTC CAGGCCCTCC AAGCAGCACC ATTGTCAGGG
2161 CAGACCTTTA CAACTCAAGC CATCTCCCAG GAAACCCTCC AGAACCTCCA GCTTCAGGCT
2221 GTTCCAAACT CTGGTCCCAT CATCATCCGG ACACCAACAG TGGGGCCCAA TGGACAGGTC
2281 AGTTGGCAGA CTCTACAGCT GCAGAACCTC CAAGTTCAGA ACCCACAAGC CCAAACAATC
2341 ACCTTAGCCC CAATGCAGGG TGTTTCCTTG GGGCAGACCA GCAGCAGCAA CACCACTCTC
2401 ACACCCATTG CCTCAGCTGC TTCCATTCCT GCTGGCACAG TCACTGTGAA TGCTGCTCAA
2461 CTCTCCTCCA TGCCAGGCCT CCAGACCATT AACCTCAGTG CATTGGGTAC TTCAGGAATC
2521 CAGGTGCACC CAATTCAAGG CCTGCCGTTG GCTATAGCAA ATGCCCCAGG TGATCATGGA
2581 GCTCAGCTTG GTCTCCATGG GGCTGGTGGT GATGGAATAC ATGATGACAC AGCAGGTGGA
2641 GAGGAAGGAG AAAACAGCCC AGATGCCCAA CCCCAAGCCG GTCGGAGGAC CCGGCGGGAA
2701 GCATGCACCT GCCCCTACTG TAAAGACAGT GAAGGAAGGG GCTCGGGGGA TCCTGGCAAA
2761 AAGAAACAGC ATATTTACCA CATCCAAGGC TGTGGGAAAG TGTATGGCAA GACCTCTCAC
2821 CTGCGGGCAC ACTTGCGCTG GCATACAGGC GAGAGGCCAT TTATGTGTAC CTGGTCATAC
2881 TGTGGGAAAC GCTTCACACG TTCGGATGAG CTACAGAGGC ACAAACGTAC ACACACAGGT
2941 GAGAAGAAAT TTGCCTGCCC TGAGTGTCCT AAGCGCTTCA TGAGGAGTGA CCACCTGTCA
3001 AAACATATCA AGACCCACCA GAATAAGAAG GGAGGCCCAG GTGTAGCTCT GAGTGTGGGC
3061 ACTTTGCCCC TGGACAGTGG GGCAGGTTCA GAAGGCAGTG GCACTGCCAC TCCTTCAGCC
3121 CTTATTACCA CCAATATGGT AGCCATGGAG GCCATCTGTC CAGAGGGCAT TGCCCGTCTT
3181 GCCAACAGTG GCATCAACGT CATGCAGGTG GCAGATCTGC AGTCCATTAA TATCAGTGGC
3241 AATGGCTTCT GAGGTACCGC GGCCGCGGGG ATCCAGACAT GATAAGATAC ATTGATGAGT
3301 TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG
3361 CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA
3421 TTCATTTTAT GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT TTTTTCGGAT CCTCTAGAGT
3481 CGATCTGCAG GCATGCTAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT
3541 GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG
3601 GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT
3661 CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT
3721 TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC
3781 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG
3841 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG
3901 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC
3961 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG
4021 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT
4081 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG
4141 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT
4201 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC
4261 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT
4321 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC
4381 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA
4441 CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT
4501 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC
4561 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT
4621 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC
4681 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG
4741 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG
4801 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC
4861 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA
4921 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG
4981 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT
5041 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA
5101 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG
5161 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA
5221 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT
5281 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA
5341 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT
5401 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT
5461 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA
5521 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT
5581 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC
5641 GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC ATGACATTAA
5701 CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT GATGACGGTG
5761 AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG
5821 GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA
5881 ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC
5941 ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC GCCATTCAGG CTGCGCAACT
6001 GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT
6061 GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA
6121 CGACGGCCAG T
