真核编辑质粒

AIO-mCherry CRISPR荧光双靶点基因编辑质粒CAS9(D10A) 双sgRNA 红色荧光

发布时间:2018-12-01 12:56:03 | 浏览次数：

测序及更多信息：https://pan.baidu.com/s/1-Ovf1Jz0SyBF8CcWYOev-w?pwd=hedg

采购地址：淘宝店铺地址

AIO-mCherry CRISPR荧光双靶点基因编辑质粒CAS9(D10A) 双sgRNA 红色荧光

PURPOSE

(Empty Backbone) All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

参考文献

CRISPR-Cas9(D10A) nickase-based genotypic and phenotypic screening to enhance genome editing. Chiang TW, le Sage C, Larrieu D, Demir M, Jackson SP. Sci Rep. 2016 Apr 15;6:24356. doi: 10.1038/srep24356. 10.1038/srep24356 PubMed 27079678