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原核编辑质粒

pCas 基因敲除载体 原核载体 CRISPR/Cas9载体
发布时间:2019-02-09 12:52:50 | 浏览次数:


测序及更多信息: https://pan.baidu.com/s/16daYQrz_iCaF_gwgj9MhJw 密码:228a

采购地址:淘宝店铺地址

载体名称:

pCas

质粒类型:

CRISPR/Cas9载体;基因敲除载体;原核载体

高拷贝/低拷贝:

低拷贝

克隆方法:

限制性内切酶,多克隆位点

启动子:

native cas9 promoter

载体大小:

12545 bp

5' 测序引物及序列:

pENTR1A-3 (GTAACATCAGAGATTTTGAGACAC)

3' 测序引物及序列:

AraC-F-New (GATGTAGCCGTCAAGTTGTCA)

载体标签:

NLSHis8 (C ter)

载体抗性:

卡那霉素(Kanamycin)

筛选标记:

--

宿主细胞(系):

细菌

备注:

Must be grown at 30℃ ! Vector has temperature-sensitive replication (RepA101ts)

稳定性:

稳表达

组成型/诱导型:

诱导型

病毒/非病毒:

非病毒

 

 






参考文献:

Jiang Y, Chen B, Duan C, et al. Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 system[J]. Applied and environmental microbiology, 2015, 81(7): 2506-2514.

 

 

全序列

1       GATCTCAAAA AAAGCACCGA CTCGGTGCCA CTTTTTCAAG TTGATAACGG ACTAGCCTTA
61       TTTTAACTTG CTATTTCTAG CTCTAAAACC TGGTAACAGG ATTAGCAGAT GTGTGAAATT
121      GTTATCCGCT CACAATTCCA CACATTATAC GAGCCGGATG ATTAATTGTC AACAGCTCAT
181      TTCAGAATAT TTGCCAGAAC CGTTATGATG TCGGCGCAAA AAACATTATC CAGAACGGGA
241      GTGCGCCTTG AGCGACACGA ATTATGCAGT GATTTACGAC CTGCACAGCC ATACCACAGC
301      TTCCGATGGC TGCCTGACGC CAGAAGCATT GGTGCACCGT GCAGTCGATG ATAAGCTGTC
361      AAACCAGATC AATTCGCGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA
421      GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG
481      TTTGCGTATT GGGCGCCAGG GTGGTTTTTC TTTTCACCAG TGAGACGGGC AACAGCTGAT
541      TGCCCTTCAC CGCCTGGCCC TGAGAGAGTT GCAGCAAGCG GTCCACGCTG GTTTGCCCCA
601      GCAGGCGAAA ATCCTGTTTG ATGGTGGTTG ACGGCGGGAT ATAACATGAG CTGTCTTCGG
661      TATCGTCGTA TCCCACTACC GAGATATCCG CACCAACGCG CAGCCCGGAC TCGGTAATGG
721      CGCGCATTGC GCCCAGCGCC ATCTGATCGT TGGCAACCAG CATCGCAGTG GGAACGATGC
781      CCTCATTCAG CATTTGCATG GTTTGTTGAA AACCGGACAT GGCACTCCAG TCGCCTTCCC
841      GTTCCGCTAT CGGCTGAATT TGATTGCGAG TGAGATATTT ATGCCAGCCA GCCAGACGCA
901      GACGCGCCGA GACAGAACTT AATGGGCCCG CTAACAGCGC GATTTGCTGG TGACCCAATG
961      CGACCAGATG CTCCACGCCC AGTCGCGTAC CGTCTTCATG GGAGAAAATA ATACTGTTGA
1021     TGGGTGTCTG GTCAGAGACA TCAAGAAATA ACGCCGGAAC ATTAGTGCAG GCAGCTTCCA
1081     CAGCAATGGC ATCCTGGTCA TCCAGCGGAT AGTTAATGAT CAGCCCACTG ACGCGTTGCG
1141     CGAGAAGATT GTGCACCGCC GCTTTACAGG CTTCGACGCC GCTTCGTTCT ACCATCGACA
1201     CCACCACGCT GGCACCCAGT TGATCGGCGC GAGATTTAAT CGCCGCGACA ATTTGCGACG
1261     GCGCGTGCAG GGCCAGACTG GAGGTGGCAA CGCCAATCAG CAACGACTGT TTGCCCGCCA
1321     GTTGTTGTGC CACGCGGTTG GGAATGTAAT TCAGCTCCGC CATCGCCGCT TCCACTTTTT
1381     CCCGCGTTTT CGCAGAAACG TGGCTGGCCT GGTTCACCAC GCGGGAAACG GTCTGATAAG
1441     AGACACCGGC ATACTCTGCG ACATCGTATA ACGTTACTGG TTTCACATTC ACCACCCTGA
1501     ATTGACTCTC TTCCGGGCGC TATCATGCCA TACCGCGAAA GGTTTTGCAC CATTCGATGG
1561     TGTCAACGTA AATGCATGCC GCTTCGCCTT CCATGGGTAT GGACAGTTTT CCCTTTGATA
1621     TGTAACGGTG AACAGTTGTT CTACTTTTGT TTGTTAGTCT TGATGCTTCA CTGATAGATA
1681     CAAGAGCCAT AAGAACCTCA GATCCTTCCG TATTTAGCCA GTATGTTCTC TAGTGTGGTT
1741     CGTTGTTTTT GCGTGAGCCA TGAGAACGAA CCATTGAGAT CATACTTACT TTGCATGTCA
1801     CTCAAAAATT TTGCCTCAAA ACTGGTGAGC TGAATTTTTG CAGTTAAAGC ATCGTGTAGT
1861     GTTTTTCTTA GTCCGTTACG TAGGTAGGAA TCTGATGTAA TGGTTGTTGG TATTTTGTCA
1921     CCATTCATTT TTATCTGGTT GTTCTCAAGT TCGGTTACGA GATCCATTTG TCTATCTAGT
1981     TCAACTTGGA AAATCAACGT ATCAGTCGGG CGGCCTCGCT TATCAACCAC CAATTTCATA
2041     TTGCTGTAAG TGTTTAAATC TTTACTTATT GGTTTCAAAA CCCATTGGTT AAGCCTTTTA
2101     AACTCATGGT AGTTATTTTC AAGCATTAAC ATGAACTTAA ATTCATCAAG GCTAATCTCT
2161     ATATTTGCCT TGTGAGTTTT CTTTTGTGTT AGTTCTTTTA ATAACCACTC ATAAATCCTC
2221     ATAGAGTATT TGTTTTCAAA AGACTTAACA TGTTCCAGAT TATATTTTAT GAATTTTTTT
2281     AACTGGAAAA GATAAGGCAA TATCTCTTCA CTAAAAACTA ATTCTAATTT TTCGCTTGAG
2341     AACTTGGCAT AGTTTGTCCA CTGGAAAATC TCAAAGCCTT TAACCAAAGG ATTCCTGATT
2401     TCCACAGTTC TCGTCATCAG CTCTCTGGTT GCTTTAGCTA ATACACCATA AGCATTTTCC
2461     CTACTGATGT TCATCATCTG AGCGTATTGG TTATAAGTGA ACGATACCGT CCGTTCTTTC
2521     CTTGTAGGGT TTTCAATCGT GGGGTTGAGT AGTGCCACAC AGCATAAAAT TAGCTTGGTT
2581     TCATGCTCCG TTAAGTCATA GCGACTAATC GCTAGTTCAT TTGCTTTGAA AACAACTAAT
2641     TCAGACATAC ATCTCAATTG GTCTAGGTGA TTTTAATCAC TATACCAATT GAGATGGGCT
2701     AGTCAATGAT AATTACTAGT CCTTTTCCTT TGAGTTGTGG GTATCTGTAA ATTCTGCTAG
2761     ACCTTTGCTG GAAAACTTGT AAATTCTGCT AGACCCTCTG TAAATTCCGC TAGACCTTTG
2821     TGTGTTTTTT TTGTTTATAT TCAAGTGGTT ATAATTTATA GAATAAAGAA AGAATAAAAA
2881     AAGATAAAAA GAATAGATCC CAGCCCTGTG TATAACTCAC TACTTTAGTC AGTTCCGCAG
2941     TATTACAAAA GGATGTCGCA AACGCTGTTT GCTCCTCTAC AAAACAGACC TTAAAACCCT
3001     AAAGGCTTAA GTAGCACCCT CGCAAGCTCG GTTGCGGCCG CAATCGGGCA AATCGCTGAA
3061     TATTCCTTTT GTCTCCGACC ATCAGGCACC TGAGTCGCTG TCTTTTTCGT GACATTCAGT
3121     TCGCTGCGCT CACGGCTCTG GCAGTGAATG GGGGTAAATG GCACTACAGG CGCCTTTTAT
3181     GGATTCATGC AAGGAAACTA CCCATAATAC AAGAAAAGCC CGTCACGGGC TTCTCAGGGC
3241     GTTTTATGGC GGGTCTGCTA TGTGGTGCTA TCTGACTTTT TGCTGTTCAG CAGTTCCTGC
3301     CCTCTGATTT TCCAGTCTGA CCACTTCGGA TTATCCCGTG ACAGGTCATT CAGACTGGCT
3361     AATGCACCCA GTAAGGCAGC GGTATCATCA ACGGGGTCTG ACGCTCAGTG GAACGAAAAC
3421     TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA
3481     AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT
3541     TACGTTTCCA CAACCAATTA ACCAATTCTG ATTAGAAAAA CTCATCGAGC ATCAAATGAA
3601     ACTGCAATTT ATTCATATCA GGATTATCAA TACCATATTT TTGAAAAAGC CGTTTCTGTA
3661     ATGAAGGAGA AAACTCACCG AGGCAGTTCC ATAGGATGGC AAGATCCTGG TATCGGTCTG
3721     CGATTCCGAC TCGTCCAACA TCAATACAAC CTATTAATTT CCCCTCGTCA AAAATAAGGT
3781     TATCAAGTGA GAAATCACCA TGAGTGACGA CTGAATCCGG TGAGAATGGC AAAAGCTTAT
3841     GCATTTCTTT CCAGACTTGT TCAACAGGCC AGCCATTACG CTCGTCATCA AAATCACTCG
3901     CATCAACCAA ACCGTTATTC ATTCGTGATT GCGCCTGAGC GAGACGAAAT ACGCGATCGC
3961     TGTTAAAAGG ACAATTACAA ACAGGAATCG AATGCAACCG GCGCAGGAAC ACTGCCAGCG
4021     CATCAACAAT ATTTTCACCT GAATCAGGAT ATTCTTCTAA TACCTGGAAT GCTGTTTTCC
4081     CGGGGATCGC AGTGGTGAGT AACCATGCAT CATCAGGAGT ACGGATAAAA TGCTTGATGG
4141     TCGGAAGAGG CATAAATTCC GTCAGCCAGT TTAGTCTGAC CATCTCATCT GTAACATCAT
4201     TGGCAACGCT ACCTTTGCCA TGTTTCAGAA ACAACTCTGG CGCATCGGGC TTCCCATACA
4261     ATCGATAGAT TGTCGCACCT GATTGCCCGA CATTATCGCG AGCCCATTTA TACCCATATA
4321     AATCAGCATC CATGTTGGAA TTTAATCGCG GCCTCGAGCA AGACGTTTCC CGTTGAATAT
4381     GGCTCATAAC ACCCCTTGTA TTACTGTTTA TGTAAGCAGA CAGTTTTATT GTTCATGATG
4441     ATATATTTTT ATCTTGTGCA ATGTAACATC AGAGATTTTG AGACACAACG TGGCTTTCCC
4501     TGCAGGGTTT GCAGTCAGAG TAGAATAGAA GTATCAAAAA AAGCACCGAC TCGGTGCCAC
4561     TTTTTCAAGT TGATAACGGA CTAGCCTTAT TTTAACTTGC TATGCTGTTT TGAATGGTTC
4621     CAACAAGATT ATTTTATAAC TTTTATAACA AATAATCAAG GAGAAATTCA AAGAAATTTA
4681     TCAGCCATAA AACAATACTT AATACTATAG AATGATAACA AAATAAACTA CTTTTTAAAA
4741     GAATTTTGTG TTATAATCTA TTTATTATTA AGTATTGGGT AATATTTTTT GAAGAGATAT
4801     TTTGAAAAAG AAAAATTAAA GCATATTAAA CTAATTTCGG AGGTCATTAA AACTATTATT
4861     GAAATCATCA AACTCATTAT GGATTTAATT TAAACTTTTT ATTTTAGGAG GCAAAAATGG
4921     ATAAGAAATA CTCAATAGGC TTAGATATCG GCACAAATAG CGTCGGATGG GCGGTGATCA
4981     CTGATGATTA TAAGGTTCCG TCTAAAAAGT TCAAGGTTCT GGGAAATACA GACCGCCACA
5041     GTATCAAAAA AAATCTTATA GGGGCTCTTT TATTTGACAG TGGAGAGACA GCGGAAGCGA
5101     CTCGTCTCAA ACGGACAGCT CGTAGAAGGT ATACACGTCG GAAGAATCGT ATTTGTTATC
5161     TACAGGAGAT TTTTTCAAAT GAGATGGCGA AAGTAGATGA TAGTTTCTTT CATCGACTTG
5221     AAGAGTCTTT TTTGGTGGAA GAAGACAAGA AGCATGAACG TCATCCTATT TTTGGAAATA
5281     TAGTAGATGA AGTTGCTTAT CATGAGAAAT ATCCAACTAT CTATCATCTG CGAAAAAAAT
5341     TGGTAGATTC TACTGATAAA GCGGATTTGC GCTTAATCTA TTTGGCCTTA GCGCATATGA
5401     TTAAGTTTCG TGGTCATTTT TTGATTGAGG GAGATTTAAA TCCTGATAAT AGTGATGTGG
5461     ACAAACTATT TATCCAGTTG GTACAAACCT ACAATCAATT ATTTGAAGAA AACCCTATTA
5521     ACGCAAGTGG AGTAGATGCT AAAGCGATTC TTTCTGCACG ATTGAGTAAA TCAAGACGAT
5581     TAGAAAATCT CATTGCTCAG CTCCCCGGTG AGAAGAAAAA TGGCTTATTT GGGAATCTCA
5641     TTGCTTTGTC ATTGGGTTTG ACCCCTAATT TTAAATCAAA TTTTGATTTG GCAGAAGATG
5701     CTAAATTACA GCTTTCAAAA GATACTTACG ATGATGATTT AGATAATTTA TTGGCGCAAA
5761     TTGGAGATCA ATATGCTGAT TTGTTTTTGG CAGCTAAGAA TTTATCAGAT GCTATTTTAC
5821     TTTCAGATAT CCTAAGAGTA AATACTGAAA TAACTAAGGC TCCCCTATCA GCTTCAATGA
5881     TTAAACGCTA CGATGAACAT CATCAAGACT TGACTCTTTT AAAAGCTTTA GTTCGACAAC
5941     AACTTCCAGA AAAGTATAAA GAAATCTTTT TTGATCAATC AAAAAACGGA TATGCAGGTT
6001     ATATTGATGG GGGAGCTAGC CAAGAAGAAT TTTATAAATT TATCAAACCA ATTTTAGAAA
6061     AAATGGATGG TACTGAGGAA TTATTGGTGA AACTAAATCG TGAAGATTTG CTGCGCAAGC
6121     AACGGACCTT TGACAACGGC TCTATTCCCC ATCAAATTCA CTTGGGTGAG CTGCATGCTA
6181     TTTTGAGAAG ACAAGAAGAC TTTTATCCAT TTTTAAAAGA CAATCGTGAG AAGATTGAAA
6241     AAATCTTGAC TTTTCGAATT CCTTATTATG TTGGTCCATT GGCGCGTGGC AATAGTCGTT
6301     TTGCATGGAT GACTCGGAAG TCTGAAGAAA CAATTACCCC ATGGAATTTT GAAGAAGTTG
6361     TCGATAAAGG TGCTTCAGCT CAATCATTTA TTGAACGCAT GACAAACTTT GATAAAAATC
6421     TTCCAAATGA AAAAGTACTA CCAAAACATA GTTTGCTTTA TGAGTATTTT ACGGTTTATA
6481     ACGAATTGAC AAAGGTCAAA TATGTTACTG AAGGAATGCG AAAACCAGCA TTTCTTTCAG
6541     GTGAACAGAA GAAAGCCATT GTTGATTTAC TCTTCAAAAC AAATCGAAAA GTAACCGTTA
6601     AGCAATTAAA AGAAGATTAT TTCAAAAAAA TAGAATGTTT TGATAGTGTT GAAATTTCAG
6661     GAGTTGAAGA TAGATTTAAT GCTTCATTAG GTACCTACCA TGATTTGCTA AAAATTATTA
6721     AAGATAAAGA TTTTTTGGAT AATGAAGAAA ATGAAGATAT CTTAGAGGAT ATTGTTTTAA
6781     CATTGACCTT ATTTGAAGAT AGGGAGATGA TTGAGGAAAG ACTTAAAACA TATGCTCACC
6841     TCTTTGATGA TAAGGTGATG AAACAGCTTA AACGTCGCCG TTATACTGGT TGGGGACGTT
6901     TGTCTCGAAA ATTGATTAAT GGTATTAGGG ATAAGCAATC TGGCAAAACA ATATTAGATT
6961     TTTTGAAATC AGATGGTTTT GCCAATCGCA ATTTTATGCA GCTGATCCAT GATGATAGTT
7021     TGACATTTAA AGAAGACATT CAAAAAGCAC AAGTGTCTGG ACAAGGCGAT AGTTTACATG
7081     AACATATTGC AAATTTAGCT GGTAGCCCTG CTATTAAAAA AGGTATTTTA CAGACTGTAA
7141     AAGTTGTTGA TGAATTGGTC AAAGTAATGG GGCGGCATAA GCCAGAAAAT ATCGTTATTG
7201     AAATGGCACG TGAAAATCAG ACAACTCAAA AGGGCCAGAA AAATTCGCGA GAGCGTATGA
7261     AACGAATCGA AGAAGGTATC AAAGAATTAG GAAGTCAGAT TCTTAAAGAG CATCCTGTTG
7321     AAAATACTCA ATTGCAAAAT GAAAAGCTCT ATCTCTATTA TCTCCAAAAT GGAAGAGACA
7381     TGTATGTGGA CCAAGAATTA GATATTAATC GTTTAAGTGA TTATGATGTC GATCACATTG
7441     TTCCACAAAG TTTCCTTAAA GACGATTCAA TAGACAATAA GGTCTTAACG CGTTCTGATA
7501     AAAATCGTGG TAAATCGGAT AACGTTCCAA GTGAAGAAGT AGTCAAAAAG ATGAAAAACT
7561     ATTGGAGACA ACTTCTAAAC GCCAAGTTAA TCACTCAACG TAAGTTTGAT AATTTAACGA
7621     AAGCTGAACG TGGAGGTTTG AGTGAACTTG ATAAAGCTGG TTTTATCAAA CGCCAATTGG
7681     TTGAAACTCG CCAAATCACT AAGCATGTGG CACAAATTTT GGATAGTCGC ATGAATACTA
7741     AATACGATGA AAATGATAAA CTTATTCGAG AGGTTAAAGT GATTACCTTA AAATCTAAAT
7801     TAGTTTCTGA CTTCCGAAAA GATTTCCAAT TCTATAAAGT ACGTGAGATT AACAATTACC
7861     ATCATGCCCA TGATGCGTAT CTAAATGCCG TCGTTGGAAC TGCTTTGATT AAGAAATATC
7921     CAAAACTTGA ATCGGAGTTT GTCTATGGTG ATTATAAAGT TTATGATGTT CGTAAAATGA
7981     TTGCTAAGTC TGAGCAAGAA ATAGGCAAAG CAACCGCAAA ATATTTCTTT TACTCTAATA
8041     TCATGAACTT CTTCAAAACA GAAATTACAC TTGCAAATGG AGAGATTCGC AAACGCCCTC
8101     TAATCGAAAC TAATGGGGAA ACTGGAGAAA TTGTCTGGGA TAAAGGGCGA GATTTTGCCA
8161     CAGTGCGCAA AGTATTGTCC ATGCCCCAAG TCAATATTGT CAAGAAAACA GAAGTACAGA
8221     CAGGCGGATT CTCCAAGGAG TCAATTTTAC CAAAAAGAAA TTCGGACAAG CTTATTGCTC
8281     GTAAAAAAGA CTGGGATCCA AAAAAATATG GTGGTTTTGA TAGTCCAACG GTAGCTTATT
8341     CAGTCCTAGT GGTTGCTAAG GTGGAAAAAG GGAAATCGAA GAAGTTAAAA TCCGTTAAAG
8401     AGTTACTAGG GATCACAATT ATGGAAAGAA GTTCCTTTGA AAAAAATCCG ATTGACTTTT
8461     TAGAAGCTAA AGGATATAAG GAAGTTAAAA AAGACTTAAT CATTAAACTA CCTAAATATA
8521     GTCTTTTTGA GTTAGAAAAC GGTCGTAAAC GGATGCTGGC TAGTGCCGGA GAATTACAAA
8581     AAGGAAATGA GCTGGCTCTG CCAAGCAAAT ATGTGAATTT TTTATATTTA GCTAGTCATT
8641     ATGAAAAGTT GAAGGGTAGT CCAGAAGATA ACGAACAAAA ACAATTGTTT GTGGAGCAGC
8701     ATAAGCATTA TTTAGATGAG ATTATTGAGC AAATCAGTGA ATTTTCTAAG CGTGTTATTT
8761     TAGCAGATGC CAATTTAGAT AAAGTTCTTA GTGCATATAA CAAACATAGA GACAAACCAA
8821     TACGTGAACA AGCAGAAAAT ATTATTCATT TATTTACGTT GACGAATCTT GGAGCTCCCG
8881     CTGCTTTTAA ATATTTTGAT ACAACAATTG ATCGTAAACG ATATACGTCT ACAAAAGAAG
8941     TTTTAGATGC CACTCTTATC CATCAATCCA TCACTGGTCT TTATGAAACA CGCATTGATT
9001     TGAGTCAGCT AGGAGGTGAC TGAAGTATAT TTTAGATGAA GATTATTTCT TAATCTAGAC
9061     ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA
9121     TTTCCCCGAA AAGTGCCACC TGCATCGATT TATTATGACA ACTTGACGGC TACATCATTC
9181     ACTTTTTCTT CACAACCGGC ACGGAACTCG CTCGGGCTGG CCCCGGTGCA TTTTTTAAAT
9241     ACCCGCGAGA AATAGAGTTG ATCGTCAAAA CCAACATTGC GACCGACGGT GGCGATAGGC
9301     ATCCGGGTGG TGCTCAAAAG CAGCTTCGCC TGGCTGATAC GTTGGTCCTC GCGCCAGCTT
9361     AAGACGCTAA TCCCTAACTG CTGGCGGAAA AGATGTGACA GACGCGACGG CGACAAGCAA
9421     ACATGCTGTG CGACGCTGGC GATATCAAAA TTGCTGTCTG CCAGGTGATC GCTGATGTAC
9481     TGACAAGCCT CGCGTACCCG ATTATCCATC GGTGGATGGA GCGACTCGTT AATCGCTTCC
9541     ATGCGCCGCA GTAACAATTG CTCAAGCAGA TTTATCGCCA GCAGCTCCGA ATAGCGCCCT
9601     TCCCCTTGCC CGGCGTTAAT GATTTGCCCA AACAGGTCGC TGAAATGCGG CTGGTGCGCT
9661     TCATCCGGGC GAAAGAACCC CGTATTGGCA AATATTGACG GCCAGTTAAG CCATTCATGC
9721     CAGTAGGCGC GCGGACGAAA GTAAACCCAC TGGTGATACC ATTCGCGAGC CTCCGGATGA
9781     CGACCGTAGT GATGAATCTC TCCTGGCGGG AACAGCAAAA TATCACCCGG TCGGCAAACA
9841     AATTCTCGTC CCTGATTTTT CACCACCCCC TGACCGCGAA TGGTGAGATT GAGAATATAA
9901     CCTTTCATTC CCAGCGGTCG GTCGATAAAA AAATCGAGAT AACCGTTGGC CTCAATCGGC
9961     GTTAAACCCG CCACCAGATG GGCATTAAAC GAGTATCCCG GCAGCAGGGG ATCATTTTGC
10021    GCTTCAGCCA TACTTTTCAT ACTCCCGCCA TTCAGAGAAG AAACCAATTG TCCATATTGC
10081    ATCAGACATT GCCGTCACTG CGTCTTTTAC TGGCTCTTCT CGCTAACCAA ACCGGTAACC
10141    CCGCTTATTA AAAGCATTCT GTAACAAAGC GGGACCAAAG CCATGACAAA AACGCGTAAC
10201    AAAAGTGTCT ATAATCACGG CAGAAAAGTC CACATTGATT ATTTGCACGG CGTCACACTT
10261    TGCTATGCCA TAGCATTTTT ATCCATAAGA TTAGCGGATC CTACCTGACG CTTTTTATCG
10321    CAACTCTCTA CTGTTTCTCC ATACCCGTTT TTTTGGGAAT TCGAGCTCTA AGGAGGTTAT
10381    AAAAAATGGA TATTAATACT GAAACTGAGA TCAAGCAAAA GCATTCACTA ACCCCCTTTC
10441    CTGTTTTCCT AATCAGCCCG GCATTTCGCG GGCGATATTT TCACAGCTAT TTCAGGAGTT
10501    CAGCCATGAA CGCTTATTAC ATTCAGGATC GTCTTGAGGC TCAGAGCTGG GCGCGTCACT
10561    ACCAGCAGCT CGCCCGTGAA GAGAAAGAGG CAGAACTGGC AGACGACATG GAAAAAGGCC
10621    TGCCCCAGCA CCTGTTTGAA TCGCTATGCA TCGATCATTT GCAACGCCAC GGGGCCAGCA
10681    AAAAATCCAT TACCCGTGCG TTTGATGACG ATGTTGAGTT TCAGGAGCGC ATGGCAGAAC
10741    ACATCCGGTA CATGGTTGAA ACCATTGCTC ACCACCAGGT TGATATTGAT TCAGAGGTAT
10801    AAAACGAATG AGTACTGCAC TCGCAACGCT GGCTGGGAAG CTGGCTGAAC GTGTCGGCAT
10861    GGATTCTGTC GACCCACAGG AACTGATCAC CACTCTTCGC CAGACGGCAT TTAAAGGTGA
10921    TGCCAGCGAT GCGCAGTTCA TCGCATTACT GATCGTTGCC AACCAGTACG GCCTTAATCC
10981    GTGGACGAAA GAAATTTACG CCTTTCCTGA TAAGCAGAAT GGCATCGTTC CGGTGGTGGG
11041    CGTTGATGGC TGGTCCCGCA TCATCAATGA AAACCAGCAG TTTGATGGCA TGGACTTTGA
11101    GCAGGACAAT GAATCCTGTA CATGCCGGAT TTACCGCAAG GACCGTAATC ATCCGATCTG
11161    CGTTACCGAA TGGATGGATG AATGCCGCCG CGAACCATTC AAAACTCGCG AAGGCAGAGA
11221    AATCACGGGG CCGTGGCAGT CGCATCCCAA ACGGATGTTA CGTCATAAAG CCATGATTCA
11281    GTGTGCCCGT CTGGCCTTCG GATTTGCTGG TATCTATGAC AAGGATGAAG CCGAGCGCAT
11341    TGTCGAAAAT ACTGCATACA CTGCAGAACG TCAGCCGGAA CGCGACATCA CTCCGGTTAA
11401    CGATGAAACC ATGCAGGAGA TTAACACTCT GCTGATCGCC CTGGATAAAA CATGGGATGA
11461    CGACTTATTG CCGCTCTGTT CCCAGATATT TCGCCGCGAC ATTCGTGCAT CGTCAGAACT
11521    GACACAGGCC GAAGCAGTAA AAGCTCTTGG ATTCCTGAAA CAGAAAGCCG CAGAGCAGAA
11581    GGTGGCAGCA TGACACCGGA CATTATCCTG CAGCGTACCG GGATCGATGT GAGAGCTGTC
11641    GAACAGGGGG ATGATGCGTG GCACAAATTA CGGCTCGGCG TCATCACCGC TTCAGAAGTT
11701    CACAACGTGA TAGCAAAACC CCGCTCCGGA AAGAAGTGGC CTGACATGAA AATGTCCTAC
11761    TTCCACACCC TGCTTGCTGA GGTTTGCACC GGTGTGGCTC CGGAAGTTAA CGCTAAAGCA
11821    CTGGCCTGGG GAAAACAGTA CGAGAACGAC GCCAGAACCC TGTTTGAATT CACTTCCGGC
11881    GTGAATGTTA CTGAATCCCC GATCATCTAT CGCGACGAAA GTATGCGTAC CGCCTGCTCT
11941    CCCGATGGTT TATGCAGTGA CGGCAACGGC CTTGAACTGA AATGCCCGTT TACCTCCCGG
12001    GATTTCATGA AGTTCCGGCT CGGTGGTTTC GAGGCCATAA AGTCAGCTTA CATGGCCCAG
12061    GTGCAGTACA GCATGTGGGT GACGCGAAAA AATGCCTGGT ACTTTGCCAA CTATGACCCG
12121    CGTATGAAGC GTGAAGGCCT GCATTATGTC GTGATTGAGC GGGATGAAAA GTACATGGCG
12181    AGTTTTGACG AGATCGTGCC GGAGTTCATC GAAAAAATGG ACGAGGCACT GGCTGAAATT
12241    GGTTTTGTAT TTGGGGAGCA ATGGCGATGA CGCATCCTCA CGATAATATC CGGGTAGGCG
12301    CAATCACTTT CGTCTACTCC GTTACAAAGC GAGGCTGGGT ATTTCCCGGC CTTTCTGTTA
12361    TCCGAAATCC ACTGAAAGCA CAGCGGCTGG CTGAGGAGAT AAATAATAAA CGAGGGGCTG
12421    TATGCACAAA GCATCTTCTG TTGAGTTAAG AACGAGTATC GAGATGGCAC ATAGCCTTGC
12481    TCAAATTGGA ATCAGGTTTG TGCCAATACC AGTAGAAACA GACGAAGAAT CCATGGGTAT
12541    GGACA
 

 

 

 

 

 

 
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